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Fucoidan Induces Cell Aggregation and Apoptosis in Osteosarcoma MG-63 Cells

Animal Cells and Systems 2016년 20권 4호 p.186 ~ 192
김혜선, 전택중,
소속 상세정보
김혜선 ( Kim Hye-Seon ) - Chosun University College of Natural Sciences Department of Biology
전택중 ( Jeon Taeck-Joong ) - Chosun University College of Natural Sciences Department of Biology

Abstract


Fucoidan, a sulfated polysaccharide found in brown algae, possesses various biological activities including anti-inflammatory and anti-cancer effects. In this study, we investigated the effects of fucoidan on the cell growth and morphology of human osteosarcoma MG-63 cells and found that fucoidan induces cell aggregation and apoptosis in osteosarcoma cells when the cells are treated with fucoidan upon seeding before becoming stably attached to the plates. Typical characteristics of apoptotic cells such as chromatin condensation and nuclear fragmentation were observed in fucoidan-treated cells. The number of annexin V-stained cells was increased by fucoidan treatment in a dose-dependent manner. Consistent with these results, cell viability and growth rate were decreased and cell spreading was inhibited in the presence of fucoidan, leading to rounded morphological changes of the cells. Our results demonstrate that fucoidan treatment results in cell aggregation through F-actin accumulation at the rounded cell cortex and apoptosis in osteosarcoma MG-63 cells.

키워드

Fucoidan; apoptosis; osteosarcoma cells; cell aggregation

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