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Fine structural reconstruction on the testicular cyst of the furrow orb weaver, Larinioides cornutus by 3D volume rendering

Animal Cells and Systems 2016년 20권 5호 p.267 ~ 275
김훈, 서재휘, 김교진, 정경훈, 문명진,
소속 상세정보
김훈 ( Kim Hoon ) - Dankook University Department of Biological Sciences
서재휘 ( Seo Jae-Hwi ) - Dankook University Department of Biological Sciences
김교진 ( Kim Kyo-Jin ) - Dankook University Department of Biological Sciences
정경훈 ( Chung Kyung-Hoon ) - Gwangju Institute of Science and Technology Bio Imaging Research Center
문명진 ( Moon Myung-Jin ) - Dankook University Department of Biological Sciences

Abstract


Morphological study on spermatids and spermatozoa have long been performed regarding various changes of cell organelles during spermiogenesis as a potential phylogenetic inference. Based on the fact that the number of germ cells per cyst increases according to a geometric series, knowing the exact number of germ cells in a certain stage may lead to the total number of sperms produced per cyst. In spiders, however, the entire process takes place in a cyst represented by a spermatogonium, producing sperms in spherical shape. It is very difficult to count the exact number of germ cells produced per cyst through a 2D image analysis. Therefore, we applied a 3D image of testicular cyst of an orb-weaving spider to visualize the exact number of germ cells produced from a cyst. In this study, 2D images obtained from serially sectioned micrographs were scanned precisely and reconstructed using a 3D-rendering technique. Finally, this research reveals that the exact number of spermatozoa produced each cyst in Larinioides cornutus appeared to be 128 (27), which indicates that a single spermatogonium undergoes five mitotic divisions and two maturing divisions (meiosis) to produce final spermatozoa.

키워드

3D reconstruction; testicular cyst; spider; Larinioides cornutus

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