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Repair of bone defects using dynamic fabricated tissue-engineered bone based on a bio-derived porous bone scaffold

Animal Cells and Systems 2016년 20권 5호 p.276 ~ 281
Song Kedong, Li Liying, Ji Lili, Li Siyuan, Mu Chunfeng, Wang Yiwei, Liu Tianqing,
소속 상세정보
 ( Song Kedong ) - Dalian University of Technology Dalian R&D Center for Stem Cell and Tissue Engineering State Key Laboratory of Fine Chemicals
 ( Li Liying ) - Dalian University of Technology Dalian R&D Center for Stem Cell and Tissue Engineering State Key Laboratory of Fine Chemicals
 ( Ji Lili ) - Dalian University of Technology Dalian R&D Center for Stem Cell and Tissue Engineering State Key Laboratory of Fine Chemicals
 ( Li Siyuan ) - Dalian University of Technology Dalian R&D Center for Stem Cell and Tissue Engineering State Key Laboratory of Fine Chemicals
 ( Mu Chunfeng ) - Anshan Iron and Steel Group Corporation
 ( Wang Yiwei ) - University of Sydney ANZAC Research Institute Burns Research Group
 ( Liu Tianqing ) - Dalian University of Technology School of Chemical Engineering State Key Laboratory of Fine Chemicals

Abstract


Fabrication and transplantation of tissue-engineered bones in a rotating wall vessel bioreactor (RWVB) was studied in the present study aiming to repair segmental bone defects. Osteoblasts were transfected with green fluorescent protein prior to seeding on bio-derived porous bone scaffolds at a density of 1?×?106?cells/mL and cultured in an RWVB for one week. For comparison, constructs were also cultured in a static condition. Morphology and structure of fabricated bones were examined using an inverted microscope, scanning electron microscope and histology analysis via hematoxylin?eosin and toluidine blue staining. Moreover, an animal model for repairing segmental bone defects of a Zelanian rabbit was used to assess the efficacy and biosafety of fabricated bones. In conclusion, tissue-engineered bones grew favorably in RWVB. In animal study, a preliminary repair of bone defects was noticed only in the experimental group after 4 weeks of implantation. Using RWVB, the fabricated tissue-engineered bone constructs were approved with better bio-capability in repairing the segmental bone defect.

키워드

Bio-derived scaffold; osteoblast; fabrication; bioreactor; repair

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