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Effects of 5,7-dihroxytryptamine administered supraspinally or spinally on the blood glucose level in D-glucose-fed and immobilization stress models

Animal Cells and Systems 2016년 20권 5호 p.289 ~ 295
박수현, 심윤범, 김성수, 이재령, Sharma Naveen, 서홍원,
소속 상세정보
박수현 ( Park Soo-Hyun ) - Hallym University College of Medicine Department of Pharmacology
심윤범 ( Sim Yun-Beom ) - Hallym University College of Medicine Department of Pharmacology
김성수 ( Kim Sung-Su ) - Hallym University College of Medicine Department of Pharmacology
이재령 ( Lee Jae-Ryeong ) - Hallym University College of Medicine Department of Pharmacology
 ( Sharma Naveen ) - Hallym University College of Medicine Department of Pharmacology
서홍원 ( Suh Hong-Won ) - Hallym University College of Medicine Department of Pharmacology

Abstract


5,7-Dihydroxytryptamine (5,7-DHT) is a neurotoxin which causes the depletion of serotonin. Moreover, the serotonergic system is the regulator of the blood glucose level. However, the role of centrally located serotonergic system in blood glucose regulation after D-glucose feed and immobilization (IMO) stress was not clearly characterized yet. Thus the present study was designed to examine the effect of 5,7-DHT administered intracerebroventricularly (i.c.v.) or intrathecally (i.t.) on the blood glucose level in D-glucose-fed and immobilization stress models. Mice were pretreated once i.c.v. or i.t. with 5,7-DHT (from 10 to 40?μg) for 3 days and D-glucose (2?g/kg) was fed orally. The blood glucose level was measured at 0, 30, 60 and 120?min after D-glucose feeding and immobilization stress initiation. We found that i.c.v. or i.t. pretreatment with 5,7-DHT attenuated the blood glucose level in both animal models. D-glucose feeding causes an increase in plasma insulin level, whereas the plasma corticosterone level was downregulated in the D-glucose-fed model. The i.c.v. or i.t. pretreatment with 5,7-DHT alone slightly increased the plasma corticosterone level. In addition, the i.c.v. or i.t. pretreatment with 5,7-DHT caused a reversal of the downregulation of plasma corticosterone level induced by D-glucose feeding, whereas immobilization stress causes an increase in plasma corticosterone and insulin levels. The i.c.v or i.t. pretreatment with 5,7-DHT attenuated the immobilization stress-induced plasma corticosterone and plasma insulin levels. Our results suggest that supraspinal and spinal depletion of serotonin appears to be responsible for the downregulation of blood glucose level in both D-glucose-fed and immobilization stress models.

키워드

5,7-DHT; blood glucose; immobilization stress; OGTT; spinal?supraspinal

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