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An extract of Ulmus macrocarpa improves cellular immunity in immuno-suppressed models

Animal Cells and Systems 2016년 20권 6호 p.353 ~ 362
이선희, 이인환, 김민환, 고지수, 이상호, 황혜진, 현숙경, 강경화, 김병우, 김철민, 정경태, 이종환,
소속 상세정보
이선희 ( Lee Sun-Hee ) - Dong-Eui University Anti-Aging Research Center
이인환 ( Lee In-Hwan ) - Dong-Eui University Anti-Aging Research Center
김민환 ( Kim Min-Hwan ) - Dong-Eui University Department of Smart-Biohealth
고지수 ( Go Ji-Su ) - Dong-eui University College of Korean Medicine Department of Physiology
이상호 ( Lee Sang-Ho ) - Dong-eui University College of Korean Medicine Department of Physiology
황혜진 ( Hwang Hye-Jin ) - Dong-Eui University Department of Food & Nutrition
현숙경 ( Hyun Sook-Kyung ) - Dong-Eui University Blue-Bio Industry RIC
강경화 ( Kang Kyung-Hwa ) - Dong-eui University College of Korean Medicine Department of Physiology
김병우 ( Kim Byung-Woo ) - Dong-Eui University Blue-Bio Industry RIC
김철민 ( Kim Cheol-Min ) - Pusan National University College of Medicine Department of Biochemistry
정경태 ( Chung Kyung-Tae ) - Dong-Eui University Department of Clinical Laboratory Science
이종환 ( Lee Jong-Hwan ) - Dong-Eui University Department of Biotechnology and Bioengineering

Abstract


An extract of Ulmus macrocarpa Hance, commonly known as the large-fruited elm, has been prescribed as a traditional medicine. In this study, we aimed to investigate the cellular immune effects of U. macrocarpa stem cortex extracts on cyclophosphamide (CY)-treated splenocytes and mice. A methanol extract showed an improved survival rate of splenocytes after 72?h. The extract was successively partitioned with dichloromethane, ethyl acetate, n-butanol, and water; and the fractions so obtained were also examined for their proliferative activity. Among them, the water fraction of U. macrocarpa showed the highest proliferation of splenocytes and was used throughout the present study. We tested the survival rate of splenocytes through dose-dependent treatment of CY and the suppression of the survival effect by CY was recovered by treatment with the water extract of U. macrocarpa. To determine the mechanism involved, we examined the expression of B-cell lymphoma-extra large (Bcl-xL) anti-apoptotic protein. CY decreased Bcl-xL protein levels in resting splenocyte cultures, whereas splenocytes were exposed to water extracts of U. macrocarpa in the presence of CY; however, elevations in Bcl-xL were observed at 96?h. Mice splenocytes treated with water extract of U. macrocarpa for cellular immunity showed an increase in the activity of the mixed lymphocyte reaction (MLR), cytotoxic T lymphocytes (CTLs), and natural killer (NK) cells. In addition, mice receiving a water extract of U. macrocarpa recovered the CTL, NK, and MLR activities suppressed by CY administration. Consequently, U. macrocarpa improves the cell-mediated immune response and provides an insight on cell-based tonic materials.

키워드

Cellular immunity; CTL; natural killer cell; Ulmus macropcarpa; water extract

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