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Neuroprotective effect of Korea Red Ginseng extract on 1-methyl-4-phenylpyridinium-induced apoptosis in PC12 Cells

Animal Cells and Systems 2016년 20권 6호 p.363 ~ 368
류선, 구성대, 하기태, 김승태,
소속 상세정보
류선 ( Ryu Sun ) - Pusan National University Korean Medicine Research Center for Healthy Aging
구성대 ( Koo Sung-Tae ) - Pusan National University Korean Medicine Research Center for Healthy Aging
하기태 ( Ha Ki-Tae ) - Pusan National University Korean Medicine Research Center for Healthy Aging
김승태 ( Kim Seung-Tae ) - Pusan National University Korean Medicine Research Center for Healthy Aging

Abstract


Background: Korean Red Ginseng (KRG) is a valuable herb in Asian countries that is used as a crude substance to inhibit inflammation and to enhance vitality, longevity and immunity. The protective effects of KRG against the toxicity of 1-methyl-4-phenylpyridinium (MPP+) were investigated in vitro in the present study.

Methods: PC12 cells were pretreated with the water extract of KRG for 24?h, then incubated with MPP+ for 24?h. The growth of the cells was assessed using a live cell viability assay, the ratio of apoptotic cells was measured using flow cytometry and morphology of the apoptotic cells was detected using terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) staining. The expressions of caspase-3 and -9 were measured by quantitative real-time PCR.

Results: Pretreatment of the KRG extract increased cell viability significantly when compared with that of only MPP+-treated cells (p?
Conclusion: These results indicate that KRG treatment suppresses MPP+-induced apoptosis in PC12 cells by regulating caspase cascades, suggesting a possible role for KRG in the prevention and treatment of Parkinson’s disease.

키워드

Parkinson’s disease; 1-methyl-4-phenylpyridine (MPP+); apoptosis; Panax ginseng; PC12 cells

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