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Multifunctional antistress effects of standardized aqueous extracts from Hippophae rhamnoides L.

Animal Cells and Systems 2016년 20권 6호 p.369 ~ 383
배동혁, 김지혜, 오둘리, 김유진, 최은진, 이현미, 정명아, 이소연, 정창식, 이민재, 강남길, 이정민, 김선오,
소속 상세정보
배동혁 ( Bae Dong-Hyuck ) - Jeollanamdo Institute of Natural Resources Research
김지혜 ( Kim Ji-Hye ) - Jeollanamdo Institute of Natural Resources Research
오둘리 ( Oh Dool-Ri ) - Jeollanamdo Institute of Natural Resources Research
김유진 ( Kim Yu-Jin ) - Jeollanamdo Institute of Natural Resources Research
최은진 ( Choi Eun-Jin ) - Jeollanamdo Institute of Natural Resources Research
이현미 ( Lee Hyun-Mi ) - Jeollanamdo Institute of Natural Resources Research
정명아 ( Jung Myung-A ) - Jeollanamdo Institute of Natural Resources Research
이소연 ( Lee So-Yeon ) - Jeollanamdo Institute of Natural Resources Research
정창식 ( Jeong Chang-Sik ) - Nutriplan Co. Ltd.
이민재 ( Lee Min-Jae ) - Nutriplan Co. Ltd.
강남길 ( Kang Nam-Gil ) - Nutriplan Co. Ltd.
이정민 ( Lee Jeong-Min ) - Kyung Hee University Department of Medical Nutrition
김선오 ( Kim Sun-Oh ) - Jeollanamdo Institute of Natural Resources Research

Abstract


This study aims to screen and identify the multi-mechanism antistress effects of an extract of Hippophae rhamnoides L. (HR) leaves on corticosterone (CORT)-induced injury, N-methyl-d-aspartate (NMDA) receptor and serotonin 6 (5-hydroxytryptamine 6, 5-HT6) receptor activity tests (in vitro), electric foot shock and forced swimming tests (FSTs) (in vivo), and tests for hippocampal CORT and monoamine levels (ex vivo), in search of active principles and underlying mechanisms of action. We confirmed that the water extract of HR (HRW) and various ethanol extracts of HR confer protective effects against CORT-induced impairments in SH-SY5Y cells and antagonistic effects on NMDA receptors and the 5-HT6 receptor by using primary cultured rat hippocampal neurons and a stable 5-HT6 receptor-expressing cell line, respectively. In addition, we confirmed the antistress effects of HRW in an electric foot shock stress model in mice and explored the underlying mechanisms of its action. We observed that HRW treatment significantly reversed the reduction in immobility times and increased climbing times in FSTs induced by electric foot shocks in the stress model. The levels of CORT, dopamine, and norepinephrine were increased, and the level of serotonin in the hippocampus was decreased in the electric foot shock stress model. The standardized HRW effectively restored abnormal CORT and monoamine levels in the hippocampus that were induced by stress. The results of the present study demonstrate that the standardized HRW produces novel multifunctional antistress effects.

키워드

Hippophae rhamnoides L; antistress; serotonin; corticosterone; monoamines

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