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Sinup is essential for the integrity of centrosomes and mitotic spindles in zebrafish embryos

Animal Cells and Systems 2017년 21권 2호 p.93 ~ 99
유경원, Maddirevula Sateesh, Kumar Ajeet, 노현주, 허태린, 이명철,
소속 상세정보
유경원 ( Yoo Kyeong-Won ) - Chungnam National University College of Bioscience and Biotechnology Department of Biological Sciences
 ( Maddirevula Sateesh ) - King Faisal Specialist Hospital and Research Center Department of Genetics
 ( Kumar Ajeet ) - Chungnam National University College of Bioscience and Biotechnology Department of Biological Sciences
노현주 ( Ro Hyun-Ju ) - Chungnam National University College of Bioscience and Biotechnology Department of Biological Sciences
허태린 ( Huh Tae-Lin ) - Kyungpook National University School of Life Sciences
이명철 ( Rhee Myung-Chull ) - Chungnam National University College of Bioscience and Biotechnology Department of Biological Sciences

Abstract


Fish lineage-specific gene, sinup [Siaz-interacting nuclear protein], modulates neural plate formation in embryogenesis and shares homology with human TPX2 protein, a member of the vertebrate mitogen-activating protein family. In spite of the presence of the TPX2 domain in Sinup, its cellular function has been unknown. As an initial approach to this question, we expressed Sinup by injecting sinup-EGFP mRNAs into zebrafish embryos at the one- to two-cell stage. First of all, Sinup-EGFP was associated with centrosomes and mitotic spindles. In particular, Sinup was localized to the spindle poles and midbody microtubules during the period between anaphase and cytokinesis. Second, various deleted mutants of Sinup-EGFP failed to be associated with the centrosomes and mitotic spindles. Third, a Sinup mutant, where the 144th Serine residue was converted to alanine, not only disturbed the mitotic spindle organization, such as multipolar spindles, fragmented spindle poles, and flattened spindles, but also arrested the cell cycle at metaphase and cell movement. Finally, Sinup is phosphorylated by Aurora A and the 144th Serine mutant of Sinup is partially phosphorylated by Aurora A kinase. We thus propose that Sinup is an essential element for the integrity of centrosomes and mitotic spindle fibers as well as for the normal process of cell cycle and cellular movement in vertebrate embryos.

키워드

Sinup; phosphorylation; centrosomes; spindle fibers

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