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Expression and regulation of Angiopoietins and their receptor Tie-2 in sika deer antler

Animal Cells and Systems 2017년 21권 3호 p.177 ~ 184
Zhang Hong-Liang, Yue Zhan-Peng, Zhang Lu, Yang Zhan-Qing, Geng Shuang, Wang Kai, Yu Hai-Fan, Guo Bin,
소속 상세정보
 ( Zhang Hong-Liang ) - Jilin University College of Veterinary Medicine
 ( Yue Zhan-Peng ) - Jilin University College of Veterinary Medicine
 ( Zhang Lu ) - Jilin University College of Veterinary Medicine
 ( Yang Zhan-Qing ) - Jilin University College of Veterinary Medicine
 ( Geng Shuang ) - Jilin University College of Veterinary Medicine
 ( Wang Kai ) - Jilin University College of Veterinary Medicine
 ( Yu Hai-Fan ) - Jilin University College of Veterinary Medicine
 ( Guo Bin ) - Jilin University College of Veterinary Medicine

Abstract


The cartilage vascularization and chondrocyte survival are essential for endochondral ossification which occurs in the process of antler growth. Angiopoietins (Ang) is a family of major angiogenic growth factors and involved in regulating the vascularization. However, the expression and regulation of Angs in the antler are still unknown. The aim of this study is to localize the expression of Ang-1, Ang-2 and their receptor Tie-2 in sika deer antler using in situ hybridization and focused on analyzing the regulation of testosterone, estrogen, all-trans-retinoic acid (ATRA) and 9cRA on their expression in antler chondrocytes. The results showed that Ang-1, Ang-2 and Tie-2 were highly expressed in antler chondrocytes. Administration of testosterone to antler chondrocytes led to a notable increase in the expression of Ang-1 and Tie-2, and a reduction in the expression of Ang-2. The similar result was also observed after estrogen treatment. In contrast, ATRA and 9cRA could inhibit the expression of Ang-1 in antler chondrocytes and heighten the expression of Ang-2. Simultaneously, ATRA could downregulate the expression of Tie-2 in antler chondrocytes at 12 and 24?h, while 9cRA upregulate the expression of Tie-2 at 3 and 6?h. Collectively, Ang-1, Ang-2 and Tie-2 are expressed in antler chondrocytes and their expression can be affected by testosterone, estrogen, ATRA and 9cRA.

키워드

Angiopoietin; testosterone; estrogen; retinoic acid; antler chondrocyte

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