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Differentiation potential of neural stem cells derived from fetal sheep

Animal Cells and Systems 2017년 21권 4호 p.233 ~ 240
Li Qian, Zhang Shuang, Zheng Yanjie, Wen Hebao, Han Xiao, Zhang Minghai, Guan Weijun,
소속 상세정보
 ( Li Qian ) - Northeast Forestry University College of Wildlife Resource
 ( Zhang Shuang ) - Harbin Institute of Physical Education Scientific Experiment Research Center
 ( Zheng Yanjie ) - Chinese Academy of Agricultural Sciences Institute of Animal Sciences
 ( Wen Hebao ) - Chinese Academy of Agricultural Sciences Institute of Animal Sciences
 ( Han Xiao ) - Chinese Academy of Agricultural Sciences Institute of Animal Sciences
 ( Zhang Minghai ) - Northeast Forestry University College of Wildlife Resource
 ( Guan Weijun ) - Chinese Academy of Agricultural Sciences Institute of Animal Sciences

Abstract


Neural stem cells (NSCs) are multipotent stem cells that can differentiate into many cell types in vitro. In this study, we isolated and established an NSC line from fetal Ovis aries. Based on the results of immunofluorescence staining, NSCs expressed Nestin, Pax6 and MAP2. Moreover, a reverse transcription?polymerase chain reaction assay was used to biologically characterize the cell line. NSCs were induced to differentiate into neurogenic cells in vitro. They expressed MAP2, glial fibrillary acidic protein (GFAP) and myelin basic protein (MBP). In this study, we successfully isolated and cultivated NSCs from the hippocampal tissue of fetal sheep. NSCs not only displayed a self-renewal capacity but also had the potential to differentiate into neurons and glial cells. This study provided valuable experimental data for NSC transplant research.

키워드

Cell culture; differentiation potential; fetal sheep; neural stem cells

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