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High-fat-diet impaired mitochondrial function of cumulus cells but improved the efficiency of parthenogenetic embryonic quality in mice

Animal Cells and Systems 2018년 22권 4호 p.243 ~ 252
Li Jingjing, Wang Shuang, Wang Bo, Wei Hao, Liu Xin, Hao Jun, Duan Yanping, Hua Jinlian, Zheng Xiaomin, Feng Xiuliang, Yan Xingrong,
소속 상세정보
 ( Li Jingjing ) - Northwest University College of Life Science
 ( Wang Shuang ) - Fourth Military Medical University Xijing Hospital Department of Experimental Surgery
 ( Wang Bo ) - Northwest University College of Life Science
 ( Wei Hao ) - Northwest University College of Life Science
 ( Liu Xin ) - Northwest University College of Life Science
 ( Hao Jun ) - Fourth Military Medical University Xijing Hospital Department of Experimental Surgery
 ( Duan Yanping ) - Fourth Military Medical University Xijing Hospital Department of Experimental Surgery
 ( Hua Jinlian ) - Northwest A&F University Biotechnology
 ( Zheng Xiaomin ) - Ningxia Medical University Key Laboratory of Fertility Preservation and Maintenance
 ( Feng Xiuliang ) - Fourth Military Medical University Xijing Hospital Department of Experimental Surgery
 ( Yan Xingrong ) - Northwest University College of Life Science

Abstract


Global human health has been compromised by high-fat diets. This study aimed to investigate the relationship between a high-fat diet and parthenogenetic embryo quality. Mice fed a high-fat or a normal diet was used as treated or control groups, respectively. Estradiol (E2), total cholesterol (TC) and total triglyceride (TG) were detected by Enzyme-Linked ImmunoSorbent Assay (ELISA). Cumulus-oocyte complexes (COCs) were collected from the mice in the treated and control groups. The ultrastructure of COCs, the expression level of genes involved in mitochondrial and nuclear functions in cumulus cells and oocytes quality were evaluated with transmission electron microscopy, real-time quantitative polymerase chain reaction (RT-PCR) and artificial parthenogenesis, respectively. The results showed that the efficiency of parthenogenetic embryonic development in vitro was significantly higher in the treated group than in the control group (p?

키워드

Embryo; high-fat diet; mouse; oocyte; parthenogenesis

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