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Multiple calmodulin genes of the Pacific abalone, Haliotis discus hannai (Mollusca: Vetigastropoda: Haliotidae)

Animal Cells and Systems 2018년 22권 5호 p.341 ~ 351
임한규, 이종규, 김건도, 정태혁,
소속 상세정보
임한규 ( Lim Han-Kyu ) - Mokpo National University Department of Marine and Fisheries Resources
이종규 ( Lee Jong-Kyu ) - Pukyong National University Department of Microbiology
김건도 ( Kim Gun-Do ) - Pukyong National University Department of Microbiology
정태혁 ( Jeong Tae-Hyug ) - Mokpo National University Department of Marine and Fisheries Resources

Abstract


In this study, we identified four canonical calmodulin genes in the Pacific abalone, Haliotis discus hannai. Their full-length cDNAs were variable in the 5′ and 3′ untranslated regions, but highly similar (91?97%) in the coding region. Each of the genes encoded 149 amino acids, with 93?97% similarity among themselves and 94?98% similarity with human CAM I. There were 54 substitutions distributed unevenly throughout the coding regions, found mostly in the third codon position. Gene structure analysis revealed that each of the calmodulin genes comprised five exons and four introns. The intron positions and phases were identical and there were no introns in the fourth exon. The corresponding introns differed in their sequences and sizes. Expression profiles of nine tissues from abalone revealed that the calmodulin genes were transcribed in common in gill and mantle tissue, but differentially in the other tissues. A phylogenetic analysis based on the amino acid sequences revealed that calmodulin C was the most common isoform in Gastropoda and calmodulin was the most diverged isoform. An in silico analysis of the calmodulin genes identified paralogous genes in other Haliotis species, indicating that gene duplication might have occurred in the last common ancestor of Haliotis.

키워드

Calmodulin; calcium signaling; expression profiling; gene structure; invertebrate

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