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TLR4-dependent effects of ISAg treatment on conventional T cell polarization in vivo

Animal Cells and Systems 2019년 23권 3호 p.184 ~ 191
이성원, 박현정, 김서현, 신수용, 김경희, 박상재, 홍석만, 전성호,
소속 상세정보
이성원 ( Lee Sung-Won ) - Sejong University Department of Integrative Bioscience and Biotechnology
박현정 ( Park Hyun-Jung ) - Sejong University Department of Integrative Bioscience and Biotechnology
김서현 ( Kim Seo-Hyun ) - Hallym University Department of Life Science
신수용 ( Shin Soo-Yong ) - Hallym University Department of Life Science
김경희 ( Kim Kyung-Hee ) - Medience Co. Ltd.
박상재 ( Park Sang-Jae ) - Medience Co. Ltd.
홍석만 ( Hong Seok-Mann ) - Sejong University Department of Integrative Bioscience and Biotechnology
전성호 ( Jeon Sung-Ho ) - Hallym University Department of Life Science

Abstract


We recently demonstrated that the polysaccharide component of the Korean medicinal herb Angelica gigas (immuno-stimulatory fraction of A. gigas; ISAg) induces anticancer effects in mice by activating natural killer (NK) and natural killer T (NKT) cells. However, it is unclear whether the use of ISAg in vivo can affect the differentiation of conventional T cells. Here, we investigated the effects of ISAg on the activation of conventional CD4+ and CD8+ T cells. We found that the administration of ISAg induced the polarization of CD4+ T cells toward the acquisition of the Th1 phenotype in vivo. Additionally, in mice treated with ISAg, CD8+ T cells produced more IFNγ than in control mice treated with PBS. Moreover, treatment with ISAg activated CD4+ and CD8+ T cells as well as NK and NKT cells, resulting in the secretion of Th1-type cytokines in a toll-like receptor 4 (TLR4)-dependent manner, implying that TLR4 is critical for an optimal Th1 response. Interestingly, ISAg treatment increased the number of Foxp3+ Treg cells, but not of Th2 cells, compared to control mice treated with PBS, indicating that ISAg possesses an immunomodulatory capacity that can control adaptive immune responses. Taken together, our results indicate that ISAg possesses a Th1-enhancing activity that could be used to treat Th2-mediated allergic immune diseases such as atopic dermatitis.

키워드

Angelica gigas; TLR4; Th1 polarization; Treg cells

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