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Histone deacetylase 6 interference protects mice against experimental stroke-induced brain injury via activating Nrf2/HO-1 pathway

Animal Cells and Systems 2019년 23권 3호 p.192 ~ 199
Li Jie, Zhao Yanping, Shi Junfeng, Ren Zhanyun, Chen Feng, Tang Wuzhuang,
소속 상세정보
 ( Li Jie ) - Jiangsu University Yixing Hospital Department of Neurology
 ( Zhao Yanping ) - Jiangsu University Yixing Hospital Department of Neurology
 ( Shi Junfeng ) - Jiangsu University Yixing Hospital Department of Neurology
 ( Ren Zhanyun ) - Jiangsu University Yixing Hospital Department of Neurology
 ( Chen Feng ) - Jiangsu University Yixing Hospital Department of Neurology
 ( Tang Wuzhuang ) - Jiangsu University Yixing Hospital Department of Neurology

Abstract


Cerebral stroke is a fatal disease with increasing incidence. The study was to investigate the role and mechanism of Histone deacetylase 6 (HDAC6) on experimental stroke-induced brain injury. The recombinant shRNA-HDAC6 or scramble shRNA lentivirus was transfected to ICR mice. Then, the ischemia/reperfusion injury (I/RI) mice were constructed using middle cerebral artery occlusion (MCAO) method. Brain TTC staining was used to determine infarct areas. Serum levels of oxidative stress-related factors were detected by enzyme linked immunosorbnent assay (ELISA). Realtime-qPCR (RT-qPCR) and Western blot were used to respectively detect mRNA and protein levels. HDAC6 was up-regulated in brain I/RI mice (MCAO group), and down-regulated again in MCAO mice transfected with shRNA-HDAC6 (MCAO?+?shRNA group). The infarct area of the MCAO mice was increased, neurological scores were higher, and serum protein levels of 3-NT, 4-HNE and 8-OHdG were higher. HDAC6 interference attenuated above effects. Though protein levels of Nrf2 and HO-1 in cytoplasm increased slightly in MCAO group, they increased significantly by HDAC6 interference. The protein levels of Nrf2 in cytoblast decreased significantly in MCAO group, and increased markedly by HDAC6 interference. HDAC6 interference protected mice against experimental stroke-induced brain injury via Nrf2/HO-1 pathway.

키워드

Histone deacetylase 6; Nrf2/HO-1; cerebral ischemia/reperfusion injury; oxidative stress

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