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Paternal bias expression of Igf2as is enhancer-dependent on the imprinting cluster of Igf2, H19 and Nctc1 in muscle cells

Animal Cells and Systems 2019년 23권 4호 p.288 ~ 293
김재영, 윤화영, Pfeifer Karl, 은복기,
소속 상세정보
김재영 ( Kim Jae-Young ) - Seoul National University College of Veterinary Medicine Department of Veterinary Internal Medicine
윤화영 ( Youn Hwa-Young ) - Seoul National University College of Veterinary Medicine Department of Veterinary Internal Medicine
 ( Pfeifer Karl ) - National Institutes of Health National Institute of Child Health and Development
은복기 ( Eun Bok-Kee ) - Korea University College of Medicine Core-Laboratory for Convergent Translational Research

Abstract


Igf2, H19, and Nctc1 are linked co-regulated genes on distal mouse chromosome 7. This locus is an important model both for studying mechanisms of monoallelic expression and for elucidating the role of cis-regulatory elements ? enhancers and insulators ? in organizing chromatin and gene expression across a large domain. In this study we characterize regulated expression of the Igf2 antisense transcript (Igf2as) in primary muscle cells. We demonstrate that Igf2as is imprinted (expressed only from the paternal chromosome). We also show that Igf2as expression during differentiation follows the same patterns as Igf2 and H19. Moreover, this expression is dependent upon the same shared enhancer element. Thus, our work shows that the imprinted cluster includes Igf2as in addition to H19, Igf2, and Nctc1.

키워드

Igf2as; Igf2; H19; imprinting; enhancer

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