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Calcineurin tax-6 regulates male ray development and counteracts with kin-29 kinase in Caenorhabditis elegans

Animal Cells and Systems 2019년 23권 6호 p.399 ~ 406
김승현, 정하나, 안주홍, 이선경,
소속 상세정보
김승현 ( Kim Seung-Hyun ) - Hanyang University Department of Life Sciences
정하나 ( Jung Ha-Na ) - Hanyang University Department of Life Sciences
안주홍 ( Ahnn Joo-Hong ) - Hanyang University Department of Life Sciences
이선경 ( Lee Sun-Kyung ) - Hanyang University Department of Life Sciences

Abstract


Phosphorylation is one of the critical protein modifications, which can lead to changing the activity of the proteins and regulating a variety of biological processes. Therefore, it is essential to properly maintain the phosphorylation level on proteins by balancing the activity of kinases and phosphatases. In this study, we report that calcineurin, a serine/threonine phosphatase, counteracts with a salt inducible kinase (SIK) to control male tail development in Caenorhabditis elegans. The counteracting regulation is cell lineage-dependent; the number of defective rays from T lineage in animals lacking calcineurin tax-6 is decreased by knock-down of SIK kin-29. This result is in contrast with the knock-down of bone marrow protein (BMP) receptor kinase sma-6, which slightly aggravates the T lineage defect. Also, sma-6 knock-down results in modest defect in ray 1 of V5 lineage in the absence of tax-6 activity. Finally, knock-down of a tyrosine phosphatase cdc-25.3 does not affect the defective ray phenotype of calcineurin tax-6 loss-of-function(lf) mutants. Altogether, these results suggest that balanced phosphorylation mediated by tax-6 and kin-29 is required for proper development of T lineage rays, and tax-6 and sma-6 may function in a parallel pathway in the developmental process of V5 lineage ray 1. This study emphasizes the elaborated developmental process of male ray formation, in which carefully coordinated expression of various genes is essential.

키워드

Caenorhabditis elegans; calcineurin; salt inducible kinase (SIK); tax-6; kin-29

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