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Honokiol ameliorates oxidative stress-induced DNA damage and apoptosis of c2c12 myoblasts by ROS generation and mitochondrial pathway

Animal Cells and Systems 2020년 24권 1호 p.60 ~ 68
박철, 최성현, 정진우, 한민호, 이혜숙, 홍수현, 김기영, 문성권, 김원재, 최영현,
소속 상세정보
박철 ( Park Cheol ) - Dong-Eui University College of Natural Sciences Department of Molecular Biology
최성현 ( Choi Sung-Hyun ) - Korea Lift College Department of System Management
정진우 ( Jeong Jin-Woo ) - Nakdonggang National Institute of Biological Resources Freshwater Bioresources Utilization Bureau
한민호 ( Han Min-Ho ) - National Marine Biodiversity Institute of Korea
이혜숙 ( Lee Hye-Sook ) - Dong-Eui University Anti-Aging Research Center
홍수현 ( Hong Su-Hyun ) - Dong-Eui University Anti-Aging Research Center
김기영 ( Kim Gi-Young ) - Jeju National University Department of Marine Life Science
문성권 ( Moon Sung-Kwon ) - Chung-Ang University Department of Food and Nutrition
김원재 ( Kim Wun-Jae ) - Chungbuk National University College of Medicine Department of Urology
최영현 ( Choi Yung-Hyun ) - Dong-Eui University Anti-Aging Research Center

Abstract


Honokiol is one of the main active components of Magnolia officinalis, and has been demonstrated to have multiple pharmacological activities against a variety of diseases. Recently, this phenolic compound is known to have antioxidant activity, but its mechanism of action remains unclear. The purpose of the current study was to evaluate the preventive effects of honokiol against oxidative stress-induced DNA damage and apoptosis in C2C12 myoblasts. The present study found that honokiol inhibited hydrogen peroxide (H2O2)-induced DNA damage and mitochondrial dysfunction, while reducing reactive oxygen species (ROS) formation. The inhibitory effect of honokiol on H2O2-induced apoptosis was associated with the up-regulation of Bcl-2 and down-regulation of Bax, thus reducing the Bax/Bcl-2 ratio that in turn protected the activation of caspase-9 and -3, and inhibition of poly (ADP-ribose) polymerase cleavage, which was associated with the blocking of cytochrome c release to the cytoplasm. Collectively, these results demonstrate that honokiol defends C2C12 myoblasts against H2O2-induced DNA damage and apoptosis, at least in part, by preventing mitochondrial-dependent pathway through scavenging excessive ROS.

키워드

Honokiol; oxidative stress; DNA damage; apoptosis; ROS

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