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Comparative analysis of immune related genes between domestic pig and germ-free minipig

Laboratory Animal Research 2020년 36권 1호 p.44 ~ 44
이주영, 김상은, 이훈택, 황정호,
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이주영 ( Lee Ju-Young ) - Korea Institute of Toxicology Animal Model Research Group
김상은 ( Kim Sang-Eun ) - Konkuk University Department of Stem Cell and Regenerative Biotechnology
이훈택 ( Lee Hoon-Taek ) - Konkuk University Department of Stem Cell and Regenerative Biotechnology
황정호 ( Hwang Jeong-Ho ) - Korea Institute of Toxicology Animal Model Research Group

Abstract


Recently, minipig has been considered as an animal model that is appropriate for human disease model to study toxicology, pharmacology, and xenotransplantation. Nevertheless, minipigs are bred in various environment according to their use. Here, we suggest that minipigs used for research should be bred in well-controlled facility, comparing immune status of pigs raised in different breeding environment. DNA microarray was performed with ear skin and placenta of Landrace domestic pigs (DPs) and Minnesota germ-free minipigs (GPs). Their immune transcriptome was analyzed by gene ontology (GO) annotation database, based on criteria of |log2 fold change| ≥1 with P?≤?0.05. As a result, we found that immune related genes in the ear skin of DPs were highly activated, compared to GPs. On the other hand, no significant s were found in the placenta. Quantitative real-time PCR (qRT-PCR) was performed in five candidate immune genes. Their fold changes were consistent with the results from DNA microarray (P?≤?0.05). In conclusion, we experimentally proved that porcine immune system was affected by different breeding environment, suggesting the importance of controlling microbes in animal room for the qualified research.

키워드

Minipig; Germ-free facility; Differentially expressed gene; Systemic immune activation

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