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Probiotic Potential of Pediococcus acidilactici and Enterococcus faecium Isolated from Indigenous Yogurt and Raw Goat Milk

Microbiology and Biotechnology Letters 2020년 48권 3호 p.276 ~ 286
Sarkar Shovon Lal, Hossain Md. Iqbal, Monika Sharmin Akter, Sanyal Santonu Kumar, Roy Pravas Chandra, Hossain Md. Anwar, Jahid Iqbal Kabir,
소속 상세정보
 ( Sarkar Shovon Lal ) - Jashore University of Science and Technology Department of Microbiology
 ( Hossain Md. Iqbal ) - Jashore University of Science and Technology Department of Microbiology
 ( Monika Sharmin Akter ) - Jashore University of Science and Technology Department of Microbiology
 ( Sanyal Santonu Kumar ) - Jashore University of Science and Technology Department of Microbiology
 ( Roy Pravas Chandra ) - Jashore University of Science and Technology Department of Microbiology
 ( Hossain Md. Anwar ) - University of Dhaka Department of Microbiology
 ( Jahid Iqbal Kabir ) - Jashore University of Science and Technology Department of Microbiology

Abstract


Probiotics are live microorganisms that, when administered in adequate amounts, confer health benefits to the host. This study was conducted for the isolation of potential lactic acid bacteria (LAB) with probiotic properties from goat milk and yogurt. Several tests were conducted in vitro using the standard procedures for evaluating the inhibitory spectra of LAB against pathogenic bacteria; tolerance to NaCl, bile salt, and phenol; hemolytic, milk coagulation, and bile salt hydrolase activities; gastrointestinal transit tolerance; adhesion properties; and antibiotic susceptibility. Among 40 LAB strains screened according to culture characteristics, five isolates exhibited antagonistic properties. Three were identified as Pediococcus acidilactici, and two were identified as Enterococcus faecium, exploiting 16S rRNA gene sequencing. All the isolates succeeded in the gastrointestinal transit tolerance assay and successively colonized mucosal epithelial cells. Based on the results of these in vitro assays, both P. acidilactici and E. faecium can be considered as potential probiotic candidates.

키워드

Probiotic; antagonistic assay; Pediococcus acidilactici; Enterococcus faecium; goat milk; 16s rRNA

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