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신규 분리된 담수미세조류 Parachlorella sp.의 지방산 생산성 향상을 위한 배지 조성 연구

Investigation on Media Composition for Cultivation of a Newly Isolated Freshwater Microalga Parachlorella sp. to Enhance Fatty Acid Productivity

Microbiology and Biotechnology Letters 2020년 48권 3호 p.328 ~ 336
Park Han-Wool, Yim Kyung-June, 민지호, 강성모, 한찬우, 이창수, 정지영, 홍승주, 이철건, 김지훈,
소속 상세정보
 ( Park Han-Wool ) - Inha University Department of Marine Science and Biological Engineering
 ( Yim Kyung-June ) - Nakdonggang National Institute of Biological Resources Microbial Research Department
민지호 ( Min Ji-Ho ) - Inha University Department of Marine Science and Biological Engineering
강성모 ( Kang Sung-Mo ) - Inha University Institute of Industrial Biotechnology
한찬우 ( Han Chan-Woo ) - Inha University Department of Marine Science and Biological Engineering
이창수 ( Lee Chang-Soo ) - Nakdonggang National Institute of Biological Resources Microbial Research Department
정지영 ( Jung Ji-Young ) - Nakdonggang National Institute of Biological Resources Microbial Research Department
홍승주 ( Hong Seong-Joo ) - Inha University Department of Marine Science and Biological Engineering
이철건 ( Lee Choul-Gyun ) - Inha University Department of Marine Science and Biological Engineering
김지훈 ( Kim Z-Hun ) - Nakdonggang National Institute of Biological Resources Microbial Research Department

Abstract


Parachlorella sp. is an efficient fatty acid producer that can be used in the production of biofuels, feeds, and fertilizers. Microalgae show varying responses to culture conditions, even those within the same species. In this study, growth and fatty acid composition of a newly isolated Parachlorella sp. from the Nakdong river of Korea in different culture media were investigated. The microalga was cultivated in 400 ml bubble column photobioreactors using BG-11, BBM, TAP, and modified TAP (MTAP) media. It was shown that using BBM led to greater fatty acid accumulation (34%), while using TAP medium led to greater biomass productivity (0.34 g/l/day). Composition of the TAP medium was modified to have the N:P ratio of BBM while also varying concentrations of N and P to improve fatty acid productivity. One of the modified TAP media, MTAP-1 (104.8 mgN/l, 135.2 mgP/l, N:P ratio = 0.77), showed the highest fatty acid concentration of 0.69 ± 0.04 g/l, while those from TAP and BBM were 0.48 ± 0.06 g/l and 0.40 ± 0.02 g/l, respectively. The results showed that microalgal fatty acid productivity could be enhanced by changing the N:P ratio and concentrations.

키워드

Biodiesel; green Microalgae; Linolenic Acid; media Selection

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