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Rhus verniciflua Stokes extract suppresses migration and invasion in human gastric adenocarcinoma AGS cells

Nutrition Research and Practice 2020년 14권 5호 p.463 ~ 477
이현숙, 정재인, 김경희, 박상재, 김은지,
소속 상세정보
이현숙 ( Lee Hyun-Sook ) - Dongseo University Department of Food Science and Nutrition
정재인 ( Jung Jae-In ) - Hallym University Regional Strategic Industry Innovation Center
김경희 ( Kim Kyeong-Hee ) - Medience Co. Ltd.
박상재 ( Park Sang-Jae ) - Medience Co. Ltd.
김은지 ( Kim Eun-Ji ) - Hallym University Regional Strategic Industry Innovation Center

Abstract


BACKGROUND/OBJECTIVES: Many studies have suggested that Rhus verniciflua Stokes (RVS) and its extract are anticancer agents. However, RVS had limited use because it contains urushiol, an allergenic toxin. By improving an existing allergen-removal extraction method, we developed a new allergen-free Rhus verniciflua Stokes extract (RVSE) with higher flavonoid content. In this study, we examined whether RVSE inhibits the ability of AGS gastric cancer cells to migrate and invade.

MATERIALS/METHODS: The flavonoids content of RVSE was analyzed by HPLC. The effects of RVSE on migration and invasion in AGS cells were analyzed by each assay kit. Matrix metalloproteinase (MMP)-9, plasminogen activator inhibitor-1 (PAI-1) and urokinase-type plasminogen activator (uPA) protein expression was analyzed by protein antibody array. The Phosphorylation of signal transducer and activator of transcription (STAT) 3 were assayed by Western blot analysis.

RESULTS: RVSE treatment with 0?100 μg/mL dose-dependently reduced the ability of AGS cells to migrate and invade. Notably, treatment with RVSE strongly inhibited the expression of MMP-9 and uPA and the phosphorylation of STAT3. In contrast, RVSE treatment dramatically increased the expression of PAI-1. These results indicate that the inhibition of MMP-9 and uPA expression and STAT3 phosphorylation and the stimulation of PAI-1 expression contributed to the decreased migration and invasion of AGS cells treated with RVSE.

CONCLUSIONS: These results suggest that RVSE may be used as a natural herbal agent to reduce gastric cancer metastasis.

키워드

Gastric cancer; metastasis; matrix metalloproteinases; urokinase-type plasminogen activator; plasminogen activator inhibitors

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