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Hypotriglyceridemic effects of brown seaweed consumption via regulation of bile acid excretion and hepatic lipogenesis in high fat diet-induced obese mice

Nutrition Research and Practice 2020년 14권 6호 p.580 ~ 592
한아름, 김재훈, 김은영, Cui Jiamei, 채인석, Zhang Guiguo, 이윤경,
소속 상세정보
한아름 ( Han A-Reum ) - Jeju National University Department of Food Science and Nutrition
김재훈 ( Kim Jae-Hoon ) - Jeju National University College of Veterinary Medicine
김은영 ( Kim Eun-Young ) - Jeju National University Department of Food Science and Nutrition
 ( Cui Jiamei ) - Jeju National University Department of Food Science and Nutrition
채인석 ( Chai In-Suk ) - Jeju National University Department of Food Science and Nutrition
 ( Zhang Guiguo ) - Shandong Agricultural University Department of Animal Nutrition
이윤경 ( Lee Yun-Kyoung ) - Jeju National University Department of Food Science and Nutrition

Abstract


BACKGROUND/OBJECTIVES: The present study aimed to further investigate the potential health beneficial effects of long-term seaweed supplementation on lipid metabolism and hepatic functions in DIO mice.

MATERIALS/METHODS: Four brown seaweeds (Undaria pinnatifida [UP], Laminaria japonica [LJ], Sargassum fulvellum [SF], or Hizikia fusiforme [HF]) were added to a high fat diet (HFD) at a 5% ratio and supplemented to C57BL/6N mice for 16 weeks. Triglycerides (TGs) and total cholesterol (TC) in the liver, feces, and plasma were measured. Fecal bile acid (BA) levels in feces were monitored. Hepatic insulin signaling- and lipogenesis-related proteins were evaluated by Western blot analysis.

RESULTS: Fasting blood glucose levels were significantly reduced in the LJ, SF, and HF groups compared to the HFD group by the end of 16-week feeding period. Plasma TG levels and hepatic lipid accumulation were significantly reduced in all 4 seaweed supplemented groups, whereas plasma TC levels were only suppressed in the UP and HF groups compared to the HFD group. Fecal BA levels were significantly elevated by UP, LJ, and SF supplementation compared to HFD feeding only. Lastly, regarding hepatic insulin signaling-related proteins, phosphorylation of 5′-AMP-activated protein kinase was significantly up-regulated by all 4 types of seaweed, whereas phosphorylation of protein kinase B was up-regulated only in the SF and HF groups. Lipogenesis-related proteins in the liver were effectively down-regulated by HF supplementation in DIO mice.

CONCLUSIONS: Brown seaweed consumption showed hypotriglyceridemic effects in the prolonged DIO mouse model. Specifically, combinatory regulation of BA excretion and lipogenesis-related proteins in the liver by seaweed supplementation contributed to the reduction of plasma and hepatic TG levels, which inhibited hyperglycemia in DIO mice. Thus, the discrepant and species-specific functions of brown seaweeds provide novel insights for the selection of future targets for therapeutic agents.

키워드

Seaweed; liver; lipogenesis; bile; mice

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