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Inhibitory Effect of Topical Cartilage Acellular Matrix Suspension Treatment on Neovascularization in a Rabbit Corneal Model

Tissue Engineering and Regenerative Medicine 2020년 17권 5호 p.625 ~ 640
Yun Hee-Woong, 최병현, 박도영, Jin Long Hao, 민병현,
소속 상세정보
 ( Yun Hee-Woong ) - Ajou University Department of Molecular Science and Technology
최병현 ( Choi Byung-Hyune ) - Inha University College of Medicine Department of Biomedical Sciences
박도영 ( Park Do-Young ) - Ajou University School of Medicine Department of Orthopaedic Surgery
 ( Jin Long Hao ) - Yanbian University Medical School Department of Orthopedic Surgery
민병현 ( Min Byoung-Hyun ) - Ajou University Department of Molecular Science and Technology

Abstract


Background: The extracellular matrix (ECM) of articular cartilage has an inhibitory effect on vascularization, yet clinical utilization has been technically challenging. In this study, we aimed to fabricate a biologically functional ECM powder suspension from porcine articular cartilage that inhibits neovascularization (NV).

Methods: The digested-cartilage acellular matrix (dg-CAM) was prepared by sequential processes of decellularization, enzymatic digestion and pulverization. Physicochemical properties of dg-CAM were compared with that of native cartilage tissue (NCT). Cellular interactions between human umbilical vein endothelial cells (HUVECs) and dg-CAM was evaluated with proliferation, migration and tube formation assays compared with that of type I collagen (COL) and bevacizumab, an anti-angiogenic drug. We then investigated the therapeutic potential of topical administration of dg-CAM suspension on the experimentally induced rabbit corneal NV model.

Results: The dg-CAM released a significantly larger amount of soluble proteins than that of the NCT and showed an improved hydrophilic and dispersion properties. In contrast, the dg-CAM contained a large amount of collagen, glycosaminoglycans and anti-angiogenic molecules as much as the NCT. The inhibitory effect on NV of the dg-CAM was more prominent than that of COL and even comparable to that of bevacizumab in inhibiting the HUVECs. The therapeutic potential of the dg-CAM was comparable to that of bevacizumab in the rabbit corneal NV model by efficiently inhibiting neovessel formation of the injured cornea.

Conclusion: The current study developed a dg-CAM having anti-angiogenic properties, together with water-dispersible properties suitable for topical or minimally invasive application for prevention of vessel invasion.

키워드

Cartilage; Extracellular matrix (ECM); Water dispersible; Enzymatic digestion; Anti-angiogenesis

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