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Promoting Osseointegration of Dental Implants in Dog Maxillary Sinus Floor Augmentation Using Dentin Matrix Protein 1-Transduced Bone Marrow Stem Cells

Tissue Engineering and Regenerative Medicine 2020년 17권 5호 p.705 ~ 715
Ma Dong, Wang Yuanyin, Chen Yongxiang, Yang Gang, Liu Xin,
소속 상세정보
 ( Ma Dong ) - Anhui Medical University Stomatologic Hospital and College Key Laboratory of Oral Diseases Research of Anhui Province
 ( Wang Yuanyin ) - Anhui Medical University Stomatologic Hospital and College Key Laboratory of Oral Diseases Research of Anhui Province
 ( Chen Yongxiang ) - Anhui Medical University Stomatologic Hospital and College Key Laboratory of Oral Diseases Research of Anhui Province
 ( Yang Gang ) - Anhui Medical University Stomatologic Hospital and College Key Laboratory of Oral Diseases Research of Anhui Province
 ( Liu Xin ) - Anhui Medical University Stomatologic Hospital and College Key Laboratory of Oral Diseases Research of Anhui Province

Abstract


Background: Beta-tricalcium phosphate (β-TCP) has been employed successfully as a synthetic graft material in maxillary sinus floor augmentation (MSFA) for placing dental implants. However, the lack of osteogenic and osteoinductive properties of this substitute invariably results in bone regeneration of low quality and quantity. The purpose of this study was to determine whether loading dentin matrix protein-1 (DMP1) gene-modified bone marrow mesenchymal stem cells (BMSCs) onto β-TCP promoted bone regeneration and osteointegration of dental implants in MSFA of dogs.

Methods: BMSCs were transduced with a lentiviral vector overexpressing the DMP1 gene (Lenti-DMP1) and with a lentiviral vector overexpressing enhanced green fluorescent protein (Lenti-EGFP) in vitro and were loaded into β-TCP scaffolds for autologous sinus grafting. Beagles received bilateral MSFA with four biomaterials (① Lenti-DMP1-transduced BMSCs/β-TCP, ② Lenti-EGFP-transduced BMSCs/β-TCP, ③ BMSCs/β-TCP, ④ β-TCP) and simultaneous implant placement at each sinus. Twelve weeks post operation, the maxillae were explanted, and every sinus was evaluated by radiographic observation, micro-CT and histological analysis. The osteogenic outcomes of bone regeneration and osseointegration were compared between the four groups.

Results: The sinuses grafted with Lenti-DMP1-transduced BMSCs/β-TCP constructs presented a significantly higher increase in compact radiopaque area, higher local bone mineral densities, greater bone-implant contact and greater bone density when compared to other three groups.

Conclusion: These results demonstrated that combinations of β-TCP and DMP1 gene-modified BMSCs could be used to construct tissue-engineered bone to enhance mineralization of the regenerated bone and osseointegration of dental implants in MSFA.

키워드

Bone marrow mesenchymal stem cells; Dentin matrix protein-1; Oral implant

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