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Irregular Bone Defect Repair Using Tissue-Engineered Periosteum in a Rabbit Model

Tissue Engineering and Regenerative Medicine 2020년 17권 5호 p.717 ~ 727
Zhao Lin, Zhao Junli, Yu Jia-Jia, Zhang Cangyu,
소속 상세정보
 ( Zhao Lin ) - Guangming Traditional Chinese Medicine Hospital of Pudong New Area Orthopaedic Department
 ( Zhao Junli ) - Shanghai University of Medicine and Health Sciences Zhoupu Hospital Department of Nephrology
 ( Yu Jia-Jia ) - Yuncheng Central Hospital Department of Joint Surgery
 ( Zhang Cangyu ) - Lanzhou University 2nd Hospital Orthopaedic Department

Abstract


Background: In previous studies, we succeeded in repairing a long bone defect with tissue-engineered periosteum (TEP), fabricated by incorporating rabbit mesenchymal stem cells with small intestinal submucosa. In this study, we investigated the feasibility of allogeneic irregular bone defect repair using TEP.

Methods: We performed a subtotal resection of the scapula in 36 rabbits to establish a large irregular bone defect model. The rabbits were then randomly divided into three groups (n?=?12 per group) and the defects were treated with TEP (Group 1), allogeneic deproteinized bone (DPB) (Group 2) or a hybrid of TEP and DPB (Group 3). At 4, 8, and 12 weeks after surgery, the rabbits were sacrificed, and the implants were harvested. X-ray radiographic and histological examinations were performed to detect bone healing. Ink-formaldehyde perfusion was introduced to qualitatively analyze vascularization in TEP engineered new bone.

Results: The repair of scapular defects was diverse in all groups, shown by radiographic and histological tests. The radiographic scores in Group 1 and Group 3 were significantly higher than Group 2 at 8 and 12 weeks (p?
Conclusion: We conclude that TEP is promising for the repair of large irregular bone defects. As a 3D scaffold, DPB could provide mechanical support and a shaping guide when combined with TEP. TEP engineered new bone has aboundant microvessels.

키워드

Tissue engineering; Periosteum; Irregular bone; Bone defect

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