Articular Cartilage Regeneration Utilizing Decellularized Human Placental Scaffold, Mesenchymal Stem Cells and Platelet Rich Plasma
Ozdemir Erdi, Emet Abdulsamet, Hashemihesar Ramin, Yuruker Ali Celalettin Sinan, Kilic Emine, Cetinkaya Duygu Uckan, Turhan Egemen,
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( Ozdemir Erdi ) - Hacettepe University Faculty of Medicine Department of Orthopedics and Traumatology
( Emet Abdulsamet ) - Hacettepe University Faculty of Medicine Department of Orthopedics and Traumatology
( Hashemihesar Ramin ) - Istanbul Aydin University Faculty of Medicine Department of Histology and Embryology
( Yuruker Ali Celalettin Sinan ) - Usak University Faculty of Medicine Department of Histology and Embryology
( Kilic Emine ) - Hacettepe University Center for Bioengineering and Biyomedtek
( Cetinkaya Duygu Uckan ) - Hacettepe University Center for Stem Cell Research and Development
( Turhan Egemen ) - Hacettepe University Faculty of Medicine Department of Orthopedics and Traumatology
Abstract
Background: Articular cartilage repair has been a challenge in orthopedic practice due to the limited self-regenerative capability. Optimal treatment method for cartilage defects has not been defined. We investigated the effect of decellularized human placental (DHP) scaffold, mesenchymal stem cells (MSC) and platelet-rich plasma (PRP) on hyaline cartilage regeneration in a rat model.
Methods: An osteochondral defect was created in trochlea region of the femur in all groups, bilaterally. No additional procedure was performed in control group (n?=?14). Only the DHP scaffold was applied to the P group (n?=?14). The DHP scaffold and 1?×?106 MSCs were applied to the PS group (n?=?14). The DHP scaffold and PRP were applied to the PP group (n?=?14). The DHP scaffold, 1?×?106 MSCs and PRP were applied to the PSP group (n?=?14). Outcome measures at 12 weeks included Pineda histology score and qualitative histology.
Results: The mean Pineda scores of P, PS, PP, and PSP groups were significantly better than the control group (p?=?0.031, p?=?0.002, p??0.05).
Conclusion: In conclusion, the DHP scaffold appears to be a promising scaffold on hyaline cartilage regeneration. The augmentation of DHP scaffold with MSCs and PRP combinations did not enhance its efficacy on articular cartilage regeneration.
키워드
Cartilage; Placenta decellularization; Platelet-rich plasma; Mesenchymal stem cells
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