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Articular Cartilage Regeneration Utilizing Decellularized Human Placental Scaffold, Mesenchymal Stem Cells and Platelet Rich Plasma

Tissue Engineering and Regenerative Medicine 2020년 17권 6호 p.901 ~ 908
Ozdemir Erdi, Emet Abdulsamet, Hashemihesar Ramin, Yuruker Ali Celalettin Sinan, Kilic Emine, Cetinkaya Duygu Uckan, Turhan Egemen,
소속 상세정보
 ( Ozdemir Erdi ) - Hacettepe University Faculty of Medicine Department of Orthopedics and Traumatology
 ( Emet Abdulsamet ) - Hacettepe University Faculty of Medicine Department of Orthopedics and Traumatology
 ( Hashemihesar Ramin ) - Istanbul Aydin University Faculty of Medicine Department of Histology and Embryology
 ( Yuruker Ali Celalettin Sinan ) - Usak University Faculty of Medicine Department of Histology and Embryology
 ( Kilic Emine ) - Hacettepe University Center for Bioengineering and Biyomedtek
 ( Cetinkaya Duygu Uckan ) - Hacettepe University Center for Stem Cell Research and Development
 ( Turhan Egemen ) - Hacettepe University Faculty of Medicine Department of Orthopedics and Traumatology

Abstract


Background: Articular cartilage repair has been a challenge in orthopedic practice due to the limited self-regenerative capability. Optimal treatment method for cartilage defects has not been defined. We investigated the effect of decellularized human placental (DHP) scaffold, mesenchymal stem cells (MSC) and platelet-rich plasma (PRP) on hyaline cartilage regeneration in a rat model.

Methods: An osteochondral defect was created in trochlea region of the femur in all groups, bilaterally. No additional procedure was performed in control group (n?=?14). Only the DHP scaffold was applied to the P group (n?=?14). The DHP scaffold and 1?×?106 MSCs were applied to the PS group (n?=?14). The DHP scaffold and PRP were applied to the PP group (n?=?14). The DHP scaffold, 1?×?106 MSCs and PRP were applied to the PSP group (n?=?14). Outcome measures at 12 weeks included Pineda histology score and qualitative histology.

Results: The mean Pineda scores of P, PS, PP, and PSP groups were significantly better than the control group (p?=?0.031, p?=?0.002, p??0.05).

Conclusion: In conclusion, the DHP scaffold appears to be a promising scaffold on hyaline cartilage regeneration. The augmentation of DHP scaffold with MSCs and PRP combinations did not enhance its efficacy on articular cartilage regeneration.

키워드

Cartilage; Placenta decellularization; Platelet-rich plasma; Mesenchymal stem cells

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