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Effect of Multiple Mutations at the Conserved TAT Sequence of T& Promoter s on their trnscription Efficiency

Molecules and Cells 1992년 2권 3호 p.349 ~ 352
김성수, Kang Chnagwon,
소속 상세정보
김성수 ( Kim Sung-Su ) - Korea Advanced Institute of Science and Technology Department of Life Science
 ( Kang Chnagwon ) - Korea Advanced Institute of Science and Technology Department of Life Science

Abstract


The calsequestrin gene of Caenorhabditis elegans is expressed in body-wall muscle cells during muscle development.
In order to study the body-wall muscle specific regulation of the calsequestrin gene expression, approximately 2kb upstream sequences of the calse-questrin gene were analyzed.
Transcriptional fusion constructs utilizing green fluorescent protein as a reporter gene were made and microinjected to produce germ-line transformed transgenic C.elegans.
The expression of green fluorescent protein was observed in the body-wall muscles of live transgenic animals under fluorescence microscopy.
Deletion analyses of upstream sequences have revealed a putative promoter sequence and a regulatory element which appeared to enhance reporter gene expression.
Both sequence elements are juxtaposed to constitute a 260 bp regulatory region approximately 260 bp upstream from the putative translational initiation codon.
Several possible binding sites for transcription factors were identified including the sites for YY1 and NF-W2, a muscle specific zinc finger transcription factor, and an ubiquitous enhancer binding protein, respectively.
Interestingly, this region also contains a 20 bp sequence element identical to those found in the mouse dystrophin gene, which suggests a possible role of this regulatory region in muscle specific gene regulation.

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