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Aryl Hydrocarbon Receptor and Autophagy-Related Protein Microtubule-Associated Protein Light Chain 3 Expression in Psoriasis

Annals of Dermatology 2021년 33권 2호 p.138 ~ 146
김정은, 김혜란, 강석영, 정민재, 허남훈, 이현주, 류애리, 김혜원, 박천욱, 정보영,
소속 상세정보
김정은 ( Kim Jung-Eun ) - Soonchunhyang University College of Medicine Soonchunhyang University Cheonan Hospital Department of Dermatology
김혜란 ( Kim Hye-Ran ) - Hallym University College of Medicine Kangnam Sacred Heart Hospital Department of Dermatology
강석영 ( Kang Seok-Young ) - Hallym University College of Medicine Kangnam Sacred Heart Hospital Department of Dermatology
정민재 ( Jung Min-Je ) - Hallym University College of Medicine Kangnam Sacred Heart Hospital Department of Dermatology
허남훈 ( Heo Nam-Hun ) - Soonchunhyang University Cheonan Hospital Clinical Trial Center
이현주 ( Lee Hyun-Ju ) - Soonchunhyang University College of Medicine Soonchunhyang University Cheonan Hospital Department of Pathology
류애리 ( Ryu Ae-Li ) - Soonchunhyang University College of Medicine Soonchunhyang University Cheonan Hospital Department of Obstetrics and Gynecology
김혜원 ( Kim Hye-One ) - Hallym University College of Medicine Kangnam Sacred Heart Hospital Department of Dermatology
박천욱 ( Park Chun-Wook ) - Hallym University College of Medicine Kangnam Sacred Heart Hospital Department of Dermatology
정보영 ( Chung Bo-Young ) - Hallym University College of Medicine Kangnam Sacred Heart Hospital Department of Dermatology

Abstract


Background: The aryl hydrocarbon receptor (AHR) and autophagy are both important to maintain skin homeostasis. However, they are also involved in skin disorders. So far, their roles in psoriasis pathogenesis are unknown.

Objective: We studied the immunohistochemical and gene expression of AHR, CYP1A1, and microtubule-associated protein light chain 3 (LC3) in lesional skin of psoriasis patients to determine correlations among them.

Methods: We included 24 psoriasis patients and ten healthy volunteers. Skin biopsies were collected. AHR, CYP1A1, and LC3 protein expression was examined by immunohistochemistry, immunofluorescence, and western blotting. AHR, CYP1A1, LC3, ATG5, BECN1 and Nrf2 mRNA levels were measured by quantitative polymerase chain reaction.

Results: AHR and CYP1A1 protein expression were higher in psoriasis lesional skin than in normal skin. LC3 protein expression was lower in psoriasis lesions than in normal controls. AHR and CYP1A1 protein expression in psoriasis lesions showed significant positive correlations with mean epidermal thickness and inflammatory cell density. Significant negative correlations were noted between LC3 protein expression in psoriasis lesions and the mean epidermal thickness or inflammatory cell density. A significant negative correlation was found between AHR and LC3 expression in psoriatic skin. AHR, CYP1A1 and Nrf2 mRNA expression were upregulated while LC3, ATG5, and BECN1 mRNA were down-regulated, in psoriatic lesional skin compared with normal controls.

Conclusion: AHR and autophagy could play a role in psoriasis pathogenesis by modifying epidermal hyperproliferation and inflammation. AHR and autophagy regulation are potential therapeutic targets in chronic inflammatory skin diseases.

키워드

Autophagy; Cytochrome P-450 CYP1A1; Psoriasis; Receptors; aryl hydrocarbon

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