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Purification of the Clonorchis Antigen using a Diethylaminoethyl-Sephadex A50 Column

Yonsei Medical Journal 1963년 4권 1호 p.43 ~ 50
김제현 ( Kim Je-Hyun ) - 연세대학교 의과대학 생화학교실

 ( Blackwell R. Q. ) - US Natal Medical Research Institute-2

Abstract


Antigens for the diagnosis of clonorchiasis have been isolated from the adult Clonorchis sinensis. Following extracttion with a phosphate-citrate buffer solution, ultrasonic disintegration, and defatting with a chloroform-methanol mixture, the antigens were fractionated in a Diethylaminoethyl-Sephadex (DEAE-Sephadex) A 50 column using sodium chloride eluates. The antigens were evaluated by carrying out both skin tests and stool examinations to determine their sensitivity, specificity and cross reaction. These DEAE fractionated antigens show less cross reaction than Melcher’s antigen. DEAE fractions were protein(280mμ peak) and nucleoprotein (260mμ peak) as indicated by spectrophotometry.

These purified fractions represented by the 260mμ and 280mμ peaks were not free of cross reactions nor were they completely specific for clonorchiasis. It is therefore suggested that further attempts be made to eliminate cross reactions and to enhance specificity by a more detailed and complete method of purification or of preparation of the chemical components.

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