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Inhibition of Long Noncoding RNA SNHG15 Ameliorates Hypoxia/Ischemia-Induced Neuronal Damage by Regulating miR-302a-3p/STAT1/NF-κB Axis

Yonsei Medical Journal 2021년 62권 4호 p.325 ~ 337
Hu Chunting, Li Chen, Ma Qiaoya, Wang Ruili, He Ya, Wang Hui, Luo Guogang,
소속 상세정보
 ( Hu Chunting ) - Xi’an Jiaotong University Second Affiliated Hospital Department of Geriatrics Neurology
 ( Li Chen ) - Xi’an Jiaotong University Second Affiliated Hospital Department of Geriatrics Neurology
 ( Ma Qiaoya ) - Xi’an Jiaotong University Second Affiliated Hospital Department of Geriatrics Neurology
 ( Wang Ruili ) - Xi’an Jiaotong University Second Affiliated Hospital Department of Geriatrics Neurology
 ( He Ya ) - Xi’an Jiaotong University Second Affiliated Hospital Department of Geriatrics Neurology
 ( Wang Hui ) - Xi’an Jiaotong University Second Affiliated Hospital Department of Geriatrics Neurology
 ( Luo Guogang ) - Xi’an Jiaotong University First Affiliated Hospital Department of Neurology

Abstract


Purpose: Ischemic brain injury results in high mortality and serious neurologic morbidity. Here, we explored the role of SNHG15 in modulating neuronal damage and microglial inflammation after ischemia stroke.

Materials and Methods: The hypoxia/ischemia models were induced by middle cerebral artery occlusion in mice and oxygen-glucose deprivation/reoxygenation (OGD/R) in vitro. Quantitative real-time PCR (qRT-PCR) and Western blot were conducted to determine the levels of SNHG15, miR-302a-3p, and STAT1/NF-κB. Moreover, gain- or loss-of functional assays of SNHG15 and miR-302a-3p were conducted. MTT assay was used to evaluate the viability of HT22 cells, and the apoptotic level was determined by flow cytometry. Furthermore, enzyme-linked immunosorbent assay was performed to detect oxidative stress and inflammatory mediators in the ischemia cortex and OGD/R-treated BV2 microglia.

Results: The SNHG15 and STAT1/NF-κB pathways were both distinctly up-regulated, while miR-302a-3p was notably down-regulated in the ischemia cortex. Additionally, overexpressing SNHG15 dramatically enhanced OGD/R-mediated neuronal apoptosis as well as the expression of oxidative stress and inflammation factors from microglia. In contrast, knocking down SNHG15 or overexpressing miR-302a-3p relieved OGD/R-mediated neuronal apoptosis and microglial activation. Moreover, the rescue experiment testified that overexpressing miR-302a-3p also attenuated SNHG15 up-regulation-induced effects. In terms of the mechanisms, SNHG15 sponged miR-302a-3p and activated STAT1/NF-κB as a competitive endogenous RNA, while miR-302a-3p targeted STAT1 and negatively regulated the STAT1/NF-κB pathway.

Conclusion: SNHG15 was up-regulated in the hypoxia/ischemia mouse or cell model. The inhibition of SNHG15 ameliorates ischemia/hypoxia-induced neuronal damage and microglial inflammation by regulating the miR-302a-3p/STAT1/NF-κB pathway.

키워드

Ischemic stroke; small nucleolar RNA host gene 15; miR-302a-3p; STAT1; inflammation

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