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Usefulness of BK virus-specific interferon-γ enzyme-linked immunospot assay for predicting the outcome of BK virus infection in kidney transplant recipients

Korean Journal of Internal Medicine 2021년 36권 1호 p.164 ~ 174
배현주, Na Do-Hyun, 장지연, 박기현, 민지원, 고은정, 이혜영, 양철우, 정병하, 오은지,
소속 상세정보
배현주 ( Bae Hyun-Joo ) - Catholic University College of Medicine Graduate School
 ( Na Do-Hyun ) - Catholic University College of Medicine Seoul St. Mary’s Hospital Department of Internal Medicine
장지연 ( Chang Ji-Yeun ) - Catholic University College of Medicine Seoul St. Mary’s Hospital Department of Internal Medicine
박기현 ( Park Ki-Hyun ) - Catholic University College of Medicine Graduate School
민지원 ( Min Ji-Won ) - Catholic University College of Medicine Bucheon St. Mary’s Hospital Department of Internal Medicine
고은정 ( Ko Eun-Jeong ) - Catholic University College of Medicine Seoul St. Mary’s Hospital Department of Internal Medicine
이혜영 ( Lee Hye-Young ) - Catholic University College of Medicine Seoul St. Mary’s Hospital Department of Laboratory Medicine
양철우 ( Yang Chul-Woo ) - Catholic University College of Medicine Seoul St. Mary’s Hospital Department of Internal Medicine
정병하 ( Chung Byung-Ha ) - Catholic University College of Medicine Seoul St. Mary’s Hospital Department of Internal Medicine
오은지 ( Oh Eun-Jee ) - Catholic University College of Medicine Seoul St. Mary’s Hospital Department of Laboratory Medicine

Abstract


Background/Aims: To investigate if BK virus (BKV)-specific T cell immunity measured by an interferon-γ enzyme-linked immunospot (ELISPOT) assay can predict the outcome of BK virus infection in kidney transplant recipients (KTRs).

Methods: We included 68 KTRs with different viremia status (no viremia [n = 17], BK viremia [n = 27], and cleared viremia [n = 24]) and 44 healthy controls (HCs). The BK viremia group was divided into controller (< 3 months) and noncontroller (> 3 months) according to sustained duration of BKV infection. We compared BKV-ELISPOT results against five BKV peptides (large tumor antigen [LT], St, VP1-3).

Results: BKV-ELISPOT results were higher in three KTRs groups with different BKV infection status than the HCs group (p < 0.05). In KTR groups, they were higher in cleared viremia group than no viremia or BK viremia group. Within the BK viremia group, controller group had higher LT-ELISPOT results compared to noncontroller group (p = 0.032). Also, KTRs without BK virus-associated nephropathy (BKVN) had higher LT, St, VP1, and VP2-ELISPOT results than those with BKVN (p < 0.05).

Conclusions: BKV-ELISPOT assay may be effective in predicting clinical outcomes of BKV infection in terms of clearance of BK virus and development of BKVN.

키워드

BK virus; Kidney transplantation; Viremia; BK polyomavirus-associated nephropathy; Enzyme-linked immunospot assay

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