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긮 Kanagawa쿡 μ ϼ

Studies on the Kanagawa Type Hemolysis of Vibrio Parahaemolyticus

Ǵ 1973 14 2ȣ p.237 ~ 243
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긮ж ̶ ͱǾ ִ Kanagawa ˰  Ͽ.
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Hemolysis of Vibrio paraaemolyticus on specialty prepared media has been considered closely related to its enteropathogenicity and called the Kanagawa phenomenon. Several reports in Japan indicate that strains of V. parahaemolyticus isolated from human diarrheal stools are Kanagawa positive, whereas almost all strains isolated from marine specimens are negative. However, this is not well accepted by some workers and reports on food poisonings associated with Kanagawa negative strains are accumulating.
In the study of Kanagawa phenomenon, the author found that a considerably large proportion of this organism isolated from marine specimens was positive for this reaction. The hemolysis on Modified Wagatsuma blood agar by Kanaganwa negative strains appeared slowly and became clearly positive at 40 to 48 hrs of incubation. The addition of 0.01 M CaCl in media greatly promoted the hemolytic activity of Kanagawa negative strains with a strongly positive reaction at 24 hrs. Many factors influenced the hemolysis by both Kanagawa positive and negative strains, but no qualitative difference was noted. The difference was supposed to be quantitative and to reside in the degree of hemolytic activity. No difference was found between Kanagawa positive and negative strains as the result of feeding experiments in dogs and cats. It was supposed from these results that Kanagawa type hemolysis of V. parahaemolyticus is an expression of strongly hemolytic activity which is distinctly differentiated from the activity of weakly hemolytic strains in special media.