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Cortisone Ѧ ġ ¿ μ ڰ ϼ

Effects of Cortisone on Phagocytic Activity of Alveolar Macrophage by Electron Microscopy

Ǵ 1973 14 2ȣ p.291 ~ 302
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Abstract

cortisone ੿ ηҮ ϰ ?Ѧ ̵ Үί ܨ ׸ ߾ ܨ ܻ ڰ Ͽ ̿ .
1) ڰδ cortisone Ͽ ˵Үͧ ؿ ־ cortisone ʰ ؿ ־ װͰ ܬ춰 Ҹ ̸ ҵ ʾҴ.
2) ڰδ ؿ ־ ϰ ?ϰ ־, cortisone ϰ ؿ ־ п ڰ ?Ǿ. ׸ ؿ ٰ II osmiophilic inclusion bodyҮ Ǿ ִ Ͽ.
3) ߾ ηҮ ˿ ־ ࿡ ?ٵ fiddm II osmiophilic inclusion bodyҮ Ǵ ҵǸ, II Ү cortisone ܬ ʴ δ.

It is well known that the phagocytic action of the cells of the reticuloendothelial system play an important role in the immunological reaction and as a barrier against foreign bodies. It is also known that when the reticuloendothelial system is blockaded by cortisone, an adherence of the foreign bodies to the cell membranes of the phagocytes, an initial step of the phagocytic mechanism, and an absorption of the foreign bodies into the cytoplasms of the phagocytes are considerably disturbed.
An investigation was undertaken to study this phagocytic activity of the alveolar macrophages and phagocytic functional aspect of the alveolar lining cells when sufficient doses of cortisone were administered to experimental animals with a resultant reticuloendothelial system blockade, by light and electron microscopic examination.
Eighteen albino rats, maintained on a stock diet, weighing about 200 gm average, were divided into 3 experimental groups.
Group 1. Control group.
No intravenous injection of india ink given.
a. 3 rats, untreated.
b. 3 rats: A dose of 25 mg of cortisone acetate was given intramusculary for 3 consecutive days.
Group 2.
6 rats: A single dose of 2.0 ml of 2% india ink in normal saline was given into the each tail veins.
Group 3.
6 rats: A dose of 25mg of cortisone acetate was given intramuscularly, once a day, for 3 consecutive days. Starting on the 4th day, a 2.0ml of 2% india ink in normal saline was administered once intravenously.
The experimental rats were sacrificed each 15 minutes (3 rats) and 30 minutes (3 rats) after the treatment of india ink preparation from the group 2 and 3. The control group was sacrificed 24 hours after the last treatment of cortisone acetate. Then the lung was extirpated and inspected for carbon pigmentation of the lung tissue, followed by both light and electron microscopic examinations.
The results were as follows:
1. Light microscopic observations:
In the experimental group of rats treated with 25mg doses of cortisone acetate with a resultant reticuloendothelial system blockade followed by an india ink injection (group 3), the degree of carbon particle deposition in the lung tissue was not diminished over the group of rats treated with india ink only (group 2).
2. Electron Microscopic observations :
In the control group of rats treated with india on1y (group 2), the alveolar macrophages were very active in engulfing the carbon particles. In this group, a large quantity of carbon particles were found to be contained in phagosomes. However, in contrast to the control group, in the experimental group of rats treated with cortisone followed by an india ink injection (group 3), the phagocytic activity of the alveolar macrophages were poorly recognizable. Only a few phagosomes bearing particles were found in the alveolar macrophages.
Unexpectedly, a large quantity of carbon particles were accumulated in the osmiophilic inclusion bodies of the type alveolar cells in both the control and experimental groups.
In conclusion then, as already described above, it was found that a considerably large quantity of carbon particles were found in the osmiophilic inclusion bodies of the type alvolar cells than found in the alveolar macrophages when 2% india ink preparation was administered into the tail veins of both experimental and control group rats with or without cortisone pretreatment. This massive accumulation of carbon particles in the osmiophilic inclusion bodies of the type alveolar cells does not appear to be affected by cortisone administration with a resultant reticuloendothelial system blockade as is the case of alvelar macrophages which were significantly inhibited of their phagccytic activity by cortisone treatment.

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