Dubowski法에 依한 血糖定量
Blood Glucose Deternination by Dubowski Method
경북의대잡지 1973년 14권 2호 p.363 ~ 367
양성기, 金在植,
소속 상세정보
양성기 ( ) - 慶北大學校 醫科大學 臨床病理學敎室
金在植 ( ) - 慶北大學校 醫科大學 臨床病理學敎室
KMID : 0351619730140020363
Abstract
Dubowski法에 依한 血糖定量을 Folin-Wu法과 比較 觀察하여 다음과 같은 結論을 얻었다.
1. Dubowski法은 Folin-Wu法에 비하여 眞正한 血糖置에 近似하였으며 操作에 所要되는 時間이 顯著히 短縮되었고 操作이 매우 簡便하였다.
2. 同一人의 血液에 있어서 Dubowski法에 依한 血糖置에 Folin-Wu法 보다 25±5.5mg% 낮게 나왔다.
3. 還元性 物質의 ?響은 galactose에서 顯著하였으나 tascorbic acid 및 glycine에 대한 ?響은 輕微하였다.
4. 70mg% 以下의 低濃度와 600mg%이상의 高濃度의 glucose溶液에서 標準檢査線과 一致하지 않으나 血液 使用量을 2倍量 혹은 2倍 稀釋하여 使用하므로서 적正이 可能하였다.
5. 全般的으로 Dubowski法에 依한 血糖定量法은 簡便하고 經濟的이며 손쉽게 試藥을 얻을 수 있는 長點이 있었다.
Today we are facing to perform the gradually increasing numbers of laboratory examinations for diabetes, and then recently there has teen required more simple and accurate method for the determination of blood glucose. The author attempted to evaluate one of the newest method. Dubowski method along popular Folin-Wu method is the substitution old method for new method.
39 cases of randome samples from normal and diabetics were determined.
The principle of Dubowski method is based upon the reaction between the o-toluidine reagent in glacial acetic acid and blood glucose resulting in green color development (glycosylamine).
The maximum absorbance was taken at 630 mμ with Coleman Junior Model 6D spectrophotometer. Dubowski method was thought to be more convenient and simple as well as the result was more close to true glucose level than Folin-Wu method with the mean difference 25±5.5mg% between two methods.
Dubowski method tended to reveal considerable effects by other reducing substance, especially galactose and lactose, but not by ascorbic acid and glycin.
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