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腸炎 비브리오菌의 溶血作用의 本態에 關한 硏究

Studies on Nature of Hemolysis of Vibrio Parahaenotyticus

경북의대잡지 1974년 15권 2호 p.137 ~ 146
강상원, 全燾基,
소속 상세정보
강상원 (  ) - 경북대학교 의과대학 세균학교실
全燾基 (  ) - 慶北大學校 醫科大學 細菌學敎室

Abstract


A clearly defined hemolysis of Vibrio parahaemolyticus on specially prepared media was called as Kanagawa phenomenon and considered closely related to the enteropathogenicity. Several reports in Japan iadicated that strains of V. parahaemolyticus isolated from stools of food poisoning patients are Kanagawa hemolysis positive, whereas almost all strains from marine specimens are negative. However, reports on food poisoning cases associated with Kanagawa negative strains are accumuating, and experimental results on the relationship between this hemolysis and the enteropathogenicity are contradictory. This report describes effects of carbohydrates and othe rfactors on this hemolysis.
It was found that hemolytic factor causing Kanagawa hemolysis was produced in Wagatsuma blood agar, a specially prepared medium, and in natrient agar containing 3% NaCl, and the difference in the appearance of this hemolysis was considered to be quantitave in nature. The addition of fermentable carbohydrates is essential for the hemolysis with different results with different carbohydrates. The hemolysis was observed not only in specially prepared media but also in nutrient and trypticase soy blood agars containing 7% Nacl, but not in media containing 3% Nacl. Fermentable carbohydrates promoted the growth of V. parahaemolyticus in poor media containing 7% NaCl and lowered the pH of media.

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