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Studies on Nature of Hemolysis of Vibrio Parahaenotyticus

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Abstract


A clearly defined hemolysis of Vibrio parahaemolyticus on specially prepared media was called as Kanagawa phenomenon and considered closely related to the enteropathogenicity. Several reports in Japan iadicated that strains of V. parahaemolyticus isolated from stools of food poisoning patients are Kanagawa hemolysis positive, whereas almost all strains from marine specimens are negative. However, reports on food poisoning cases associated with Kanagawa negative strains are accumuating, and experimental results on the relationship between this hemolysis and the enteropathogenicity are contradictory. This report describes effects of carbohydrates and othe rfactors on this hemolysis.
It was found that hemolytic factor causing Kanagawa hemolysis was produced in Wagatsuma blood agar, a specially prepared medium, and in natrient agar containing 3% NaCl, and the difference in the appearance of this hemolysis was considered to be quantitave in nature. The addition of fermentable carbohydrates is essential for the hemolysis with different results with different carbohydrates. The hemolysis was observed not only in specially prepared media but also in nutrient and trypticase soy blood agars containing 7% Nacl, but not in media containing 3% Nacl. Fermentable carbohydrates promoted the growth of V. parahaemolyticus in poor media containing 7% NaCl and lowered the pH of media.

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