寒冷暴露가 닭肝 Phospoenolpyruvate Carboxykinase에 미치는 影響
Effect of Cold Exposure on Phospoenolpyruvate Carboxykinase of Chicken Liver
경북의대잡지 1977년 18권 2호 p.189 ~ 194
이두룡, 曺準承,
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이두룡 ( ) - 慶北大學校 醫科大學 生化學敎室
曺準承 ( ) - 慶北大學校 醫科大學 生化學敎室
KMID : 0351619770180020189
Abstract
This study is to observe the change of ; phosphoenolpyruvate carboxykinase (EC 4. 1. 1. 32) activity of chicken liver when it was treated with scold ,exposure. The enzyme activity was determined by means of oxaloacetate-H1~C0-s exchange reaction, and various froms of the enzyme were analysed by DEAE-sephadex chromatography.
The results obtained were summerized as follows:
The enzyme is found to be existed in cytosolic or mitochondral fraction of the liver, and enzyme of each fraction of young chicken liver has been separated into t~.vo isozymic forms by means of DFAE-sephadex column chromatography. These forms were designated as form I and II in their order of elution from the column. In adult chicken, however, from I of the enzyme has been found only, with form II showing extremely too low to be recognized as a peak.
Being placed in the cold room for 24 or 48 hours, the enzyme of chicken was relatively induced low, whereas the chromatographical profile of the enzyme carne to be changed markedly_, with form I reduced to be low and from II emerged predomin~intly. These findings suggest that isozymic forms of the enzyme were regulated independently b;y cold exposure.
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