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Purification of ginseng rare sapogenins 25-OH-PPT and its hypoglycemic, antiinflammatory and lipid-lowering mechanisms

Journal of Ginseng Research 2021년 45권 1호 p.86 ~ 97
Xu Jing, Liu Hairong, Su Guangyue, Ding Meng, Wang Wei, Lu Jincai, Bi Xiuli, Zhao Yuqing,
소속 상세정보
 ( Xu Jing ) - Shenyang Pharmaceutical University School of Functional Food and Wine
 ( Liu Hairong ) - Shenyang Pharmaceutical University School of Functional Food and Wine
 ( Su Guangyue ) - Shenyang Pharmaceutical University School of Functional Food and Wine
 ( Ding Meng ) - Shenyang Pharmaceutical University School of Functional Food and Wine
 ( Wang Wei ) - Shenyang Pharmaceutical University School of Functional Food and Wine
 ( Lu Jincai ) - Shenyang Pharmaceutical University Department of Medicinal Plant
 ( Bi Xiuli ) - Liaoning University School of Life Science
 ( Zhao Yuqing ) - Shenyang Pharmaceutical University School of Functional Food and Wine

Abstract


Background: Panax ginseng Meyer has been used as a nourishing edible herb in East Asia for thousands of years. 25-OH-PPT was first discovered as a natural rare triterpenoid saponin in ginseng stems and leaves by our group. Research found that it showed strong inhibitory effects on α-glucosidase and protein tyrosine phosphatase 1B, and protected cardiocytes (H9c2) through PI3K/Akt pathway.

Methods: In the research, in order to optimize the 25-OH-PPT enrichment process, optimal macroporous resins and optimal purification conditions were studied. Meanwhile, the hypoglycemic effect and mechanism of 25-OH-PPT were evaluated by using STZ to establish insulin-dependent diabetic mice and the spontaneous type 2 diabetes DB/DB mice.

Results and Conclusion: Research found that 25-OH-PPT can reduce blood glucose and enhance glucose tolerance in STZ model mice. It increases insulin sensitivity by upregulating GLUT4 and AMPK in skeletal muscle, and activating insulin signaling pathways. In DB/DB mice, 25-OH-PPT achieves hypoglycemic effects mainly by activating the insulin signaling pathway. Meanwhile, through the influence of liver inflammatory factors and lipids in serum, it can be seen that 25-OH-PPT has obvious anti-inflammatory and lipid-lowering effects. These results provide new insights into the study of ginseng as a functional food.

키워드

Ginseng; Insulin resistance; Macroporous resin; T2DM

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