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Quantitative aspects of the hydrolysis of ginseng saponins: Application in HPLC-MS analysis of herbal products

Journal of Ginseng Research 2021년 45권 2호 p.246 ~ 253
Abashev Mikhail, Stekolshchikova Elena, Stavrianidi Andrey,
소속 상세정보
 ( Abashev Mikhail ) - Lomonosov Moscow State University Chemistry Department
 ( Stekolshchikova Elena ) - Skolkovo Institute of Science and Technology Center for Neurobiology and Brain Restoration
 ( Stavrianidi Andrey ) - Lomonosov Moscow State University Chemistry Department

Abstract


Background: Ginseng is one of the most valuable herbal supplements. It is challenging to perform quality control of ginseng products due to the diversity of bioactive saponins in their composition. Acid or alkaline hydrolysis is often used for the structural elucidation of these saponins and sugars in their side chains. Complete transformation of the original ginsenosides into their aglycones during the hydrolysis is one of the ways to determine a total saponin group content. The main hurdle of this approach is the formation of various by-products that was reported by many authors.

Methods: Separate HPLC assessment of the total protopanaxadiol, protopanaxatriol and ocotillol ginsenoside contents is a viable alternative to the determination of characteristic biomarkers of these saponin groups, such as ginsenoside Rf and pseudoginsenoside F11, which are commonly used for authentication of P. ginseng Meyer and P. quinquefolius L. samples respectively. Moreover, total ginsenoside content is an ideal aggregated parameter for standardization and quality control of ginseng-based medicines, because it can be directly applied for saponin dosage calculation.

Results: Different hydrolysis conditions were tested to develop accurate quantification method for the elucidation of total ginsenoside contents in herbal products. Linearity, limits of quantification, limits of detection, accuracy and precision were evaluated for the developed HPLC-MS method.

Conclusion: Alkaline hydrolysis results in fewer by-products than sugar elimination in acidic conditions. An equimolar response, as a key parameter for quantification, was established for several major ginsenosides. The developed approach has shown acceptable results in the analysis of several different herbal products.

키워드

Ginsenosides; Hydrolysis; HPLC-MS; Quality control; Herbal products

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