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Ginsenoside Rb1 inhibits monoiodoacetate-induced osteoarthritis in postmenopausal rats through prevention of cartilage degradation

Journal of Ginseng Research 2021년 45권 2호 p.287 ~ 294
Aravinthan Adithan, Hossain Mohammad Amjad, 김범석, 강창원, 김남수, 황기철, 김종훈,
소속 상세정보
 ( Aravinthan Adithan ) - Chonbuk National University College of Veterinary Medicine
 ( Hossain Mohammad Amjad ) - Chonbuk National University College of Veterinary Medicine
김범석 ( Kim Bum-Seok ) - Chonbuk National University College of Veterinary Medicine
강창원 ( Kang Chang-Won ) - Chonbuk National University College of Veterinary Medicine
김남수 ( Kim Nam-Soo ) - Chonbuk National University College of Veterinary Medicine
황기철 ( Hwang Ki-Chul ) - Catholic Kwandong University College of Medicine Department of Medicine
김종훈 ( Kim Jong-Hoon ) - Chonbuk National University College of Veterinary Medicine

Abstract


Background: Ginsenoside Rb1 (G-Rb1), one of the major active compounds in Panax ginseng, has already been shown to reduce inflammation in various diseases. Osteoarthritis (OA) has traditionally been considered a degenerative disease with degradation of joint articular cartilage. However, recent studies have shown the association of inflammation with OA. In the present study, we investigated whether Rb1 had an antiinflammatory effect on monoiodoacetate (MIA)-induced OA in ovariectomized rats as a model of postmenopausal arthritis.

Methods: G-Rb1 at a dosage of 3 and 10 μg/kg body weight was administered every 3 days intraarticularly for a period of 4 weeks to observe antiarthritic effects. Diclofenac (10 mg/kg) served as a positive control.

Results: The administration of Rb1 significantly ameliorated OA inflammatory symptoms and reduced serum levels of inflammatory cytokines. Furthermore, G-Rb1 administration considerably enhanced the expression of bone morphogenetic protein-2 and collagen 2A and reduced the levels of matrix metalloproteinase-13 genes, indicating a chondroprotective effect of G-Rb1. G-Rb1 also significantly reduced the expression of several inflammatory cytokines/chemokines (interferon gamma (IFN-γ), monocyte chemoattractant protein-1 (MCP-1)/CCL-2, interleukin [IL]-1β, and IL-6). Histological analysis demonstrated that G-Rb1 significantly attenuated the pathological changes in MIA-induced OA in ovariectomized rats. Safranin O and toluidine blue staining further demonstrated that G-Rb1 effectively prevented the degradation of cartilage and glycosaminoglycans, respectively.

Conclusion: Overall, our results suggest that G-Rb1 exerts cartilage protective effect on MIA-induced ovariectomized OA rats, by inhibiting inflammatory mediators such as IL-6, IL-1β, MCP-1/CCL-2, cyclooxygenase-2 (COX-2), and prostaglandin E2 (PGE2). These results shed a light on possible therapeutic application of G-Rb1 in OA.

키워드

Ginsenoside-Rb1; Hstological analysis; Monoiodoacetate; Ovariectomy; Osteoarthritis

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