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Identification and quantification of oleanane triterpenoid saponins and potential analgesic and anti-inflammatory activities from the roots and rhizomes of Panax stipuleanatus

Journal of Ginseng Research 2021년 45권 2호 p.305 ~ 315
Shu Pan-Pan, Li Lu-Xi, He Qin-Min, Pan Jun, Li Xiao-Lei, Zhu Min, Yang Ye, Qu Yuan,
소속 상세정보
 ( Shu Pan-Pan ) - Kunming University of Science and Technology Faculty of Life Science and Technology
 ( Li Lu-Xi ) - Kunming University of Science and Technology Faculty of Life Science and Technology
 ( He Qin-Min ) - Kunming University of Science and Technology Faculty of Life Science and Technology
 ( Pan Jun ) - Yunnan Provincal Academy of Agricultural Sciences
 ( Li Xiao-Lei ) - Kunming University of Science and Technology Faculty of Life Science and Technology
 ( Zhu Min ) - Kunming University of Science and Technology Faculty of Life Science and Technology
 ( Yang Ye ) - Kunming University of Science and Technology Faculty of Life Science and Technology
 ( Qu Yuan ) - Kunming University of Science and Technology Faculty of Life Science and Technology

Abstract


Background: Panax stipuleanatus represents a folk medicine for treatment of inflammation. However, lack of experimental data does not confirm its function. This article aims to investigate the analgesic and anti-inflammatory activities of triterpenoid saponins isolated from P. stipuleanatus.

Methods: The chemical characterization of P. stipuleanatus allowed the identification and quantitation of two major compounds. Analgesic effects of triterpenoid saponins were evaluated in two models of thermal- and chemical-stimulated acute pain. Anti-inflammatory effects of triterpenoid saponins were also evaluated using four models of acetic acid?induced vascular permeability, xylene-induced ear edema, carrageenan-induced paw edema, and cotton pellet?induced granuloma in mice.

Results: Two triterpenoid saponins of stipuleanosides R1 (SP-R1) and R2 (SP-R2) were isolated and identified from P. stipuleanatus. The results showed that SP-R1 and SP-R2 significantly increased the latency time to thermal pain in the hot plate test and reduced the writhing response in the acetic acid?induced writhing test. SP-R1 and SP-R2 caused a significant decrease in vascular permeability, ear edema, paw edema, and granuloma formation in inflammatory models. Further studies showed that the levels of inflammatory mediators, nitric oxide, malondialdehyde, tumor necrosis factor-α, and interleukin 6 in paw tissues were downregulated by SP-R1 and SP-R2. In addition, the rational harvest of three- to five-year-old P. stipuleanatus was preferable to obtain a higher level of triterpenoid saponins. SP-R2 showed the highest content in P. stipuleanatus, which had potential as a chemical marker for quality control of P. stipuleanatus.

Conclusion: This study provides important basic information about utilization of P. stipuleanatus resources for production of active triterpenoid saponins.

키워드

Analgesic; Anti-inflammatory; Panax stipuleanatus; Paw edema; Stipuleanosides

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