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混濁法에 依한 아미노酸 營養輸液製劑의 微生物學的 分析에 關한 硏究

A Study on the Microbiological Assay of Amino Acid Preparation by Turbidimetric Method

공중보건잡지 1973년 10권 1호 p.1 ~ 9
윤남석,
소속 상세정보
윤남석 (  ) - 서울대학교 보건대학원

Abstract


The pressing need for more specific information on amino acid preparation gave rise to procedures involving microbiological and column chromatographic methods for individual amino acids.
This experiment was conducted to select a suitable test organism, the concentration range of each amino acid and key factors for the microbiological assay of Glycine, L-Isoleucine, L-Leucine, L-Lysine, L-Threonine and L-Valine by turbidimetric method.
In addition to the results have been compared with two methods for amino acid analysis, column chromatographic method and microbiological assay.
The results of this study were as follows;
1. Leuconostoc mesenteroides was more sensitive and responsive to increasing amount of Glycine, L-Isoleucine. L-Leucine, L-Lysine, L-Threonine and L-Valine than Lactobacillus arabinosus or Streptococcus faecalis. So Leuconostoc mesenteroides is suitable for the assay of these amino acids.
2. The concentration ranges of each amino acid in which Leuconostoc mesenteroides grew proportionally were as follows.
Glycine; 1-4mcg/ml L-Isoleucine; 1-4mcg/ml L-Leucine; 0-4mcg/ml L-Lysine; 0-10mcg/ml L-Threonine; 1-5mcg/ml L-Valine; 6-10mcg/ml
3. In the process of microbiological assay of Amino Acids by turbidimetric method such factors as a suitable test organism adoption, a suitable amino acid concentration range determination, inoculum dilution rate, incubation period and reproducibility of standard curve must be considered as key factors.
4. There was no significant difference that confidence limits at P=0.05 in both method, namely, the results of column chromatographic method for amino acid analysis agreed well with the microbiological assay.

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