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Realtime PCR법과 세균배양법을 이용한 치아우식세균 검사법 비교 연구

Caries activity test by realtime PCR method and bacterial cultural method

대한구강보건학회지 2020년 44권 3호 p.126 ~ 129
윤정희, 박지현, 조자원, 김성원,
소속 상세정보
윤정희 ( Yun Joung-Hee ) - Dankook University Graduate School of Public Health and Social Welfare Department of Oral Health
박지현 ( Park Ji-Hyeon ) - Gangneung Yeongdong College Department of Dental Hygiene
조자원 ( Cho Ja-Won ) - Dankook University College of Dentistry Department of Preventive Dentistry
김성원 ( Kim Sung-Won ) - Dankook University College of Dentistry Department of Preventive Dentistry

Abstract


Objectives: This study aimed to compare the results of conventional methodology and real-time polymerase chain reaction (PCR) for acid-producing bacteria and identify the appropriate method for caries activity testing.

Methods: Oral examination and caries activity tests, such as the CRT® Bacteria test and modified Snyder test, were conducted on 33 middle school students in Cheongju, South Korea. Pearson correlation analysis was subsequently performed with a significance level of 5%.

Results: The amount of Streptococcus mutans determined using the CRT® Bacteria test was highly correlated with the decayed teeth (DT) index (R=0.570, P<0.05); decayed, missing, and filled teeth (DMFT) index (R=0.376, P<0.05); and dental health capacity of the first permanent molar (R=―0.395, P<0.05). The amount of Streptococcus mutans determined using real-time PCR was significantly associated with the DT index (R=0.528, P<0.05), DMFT index (R=0.369, P<0.05), and dental health capacity of the first permanent molar (R=―0.426, P =0.013).

Conclusions: There was a significant correlation between the amount and the type of bacteria between the CRT® Bacteria test and real-time PCR; therefore, these tests were found to be more appropriate to determine caries activity than the modified Snyder test. This study suggests that realtime PCR is a better technique for detecting caries activities than conventional techniques, such as the CRT® Bacteria test and modified Snyder test, because it is easy to use and provides accurate results.

키워드

Caries activity test; Oral microorganism; Streptococcus mutans

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