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Cytosine Arabinoside와 Polyribonucleotides가 흰쥐 切齒齒髓組織에 미치는 影向에 關한 硏究

A STUDY OF EFFECTS OF CYTOSINE ARABINOSIDE AND POLYRIBONUCLEOTIDES ON THE PULP TISSUES OF RAT INCISORS

대한구강해부학회지 1977년 1권 1호 p.5 ~ 13
김성용,
소속 상세정보
김성용 (  ) - 서울대학교 대학원 치의학과 구강해부학 전공

Abstract


Male rats, weighing about 100gm were used throughout all experiments. TheY were divided to cytosine arabinoside group, cytosine arabinoside group receiving polyribonucleotides and control group.
Experimental animals were given an intramuscular injection of cytosine, arabinoside 50mg per kg of body weight. Polyadenylic acid (poly A) and polyuridylic acid (poly U), were used in this investigation.
Experimental rats, were given an intraperitoneal injection of poly A:U(lmg/kg) , 24 hours prior to sacrifice. The animals were sacrificed 1, 3, 5, 7, 10 and 14 days after injection of these drugs.
For determination of DNA content, the diplienylamine, reaction of Burton was used. Total protein of the pulp tissues were measured by the Folin phenol reagent.
The intensity of the subsequent color reaction was determined by absorbance at 600mμ for DNA and 750mμ for protein in the spectronic 20 spectrometer.
The results were compared with data derived from calf thymus DNA and bovine serum albumin as standard and expressed as μg of DNA and protein. To establish the relative cellularity of the pulp tissues from different groups, D A and protein content per mg of tissue were assessed.
The objectives of this study were to evaluate the effects of cytosine arabinoside group and cytosine arabinoside group receiving poly A: U on the biochemical functioning of pulp tissues in rat incisors.
The results were as follows;
1. The effects of cytosine arabinoside group and cytosine arabinoside group receiving polyribonucleotides on the body weight and weight of pulp tissues were produced significant decrease during the first 5th days followed by a gradual recovery thereafter. The body weight and pulp tissue weight of the cytosine arabinoside group receiving polyribonucleotides were higher than that not receiving polyribonucleotides.
2. The DNA and protein content in the groups receiving polyribonucleotides were higher than those not injected with nucleotide mixture.
3. The effects of cytosine arabinoside group without and with poly A:U on t he DNA and protein content of the pulp tissues were produced a considerable decrease during the first 5th days followed by gradual recovery thereafter.
4. Therefore, the results suggest that poly A:U might have a limited stimulatory effect on the DNA and protein synthesis of the pulp tissues in rat incisors. It has been shown that synthetic, polyribonucleotides, when used in an appropriate quantity were able to enhance DNA and protein synthesis in the pulp tissues of the rat incisors.

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