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血淸, 血漿및 卵白의 結晶像과 子宮頸管分泌液의 結晶像에 關한 硏究

대한산부인과학회지 1960년 3권 1호 p.27 ~ 45
김석환 (  ) - 서울대학교 醫科大學 産婦人科學敎室

申漢秀 (  ) - 서울대학교 醫科大學 産婦人科學敎室

Abstract


The crystallization in the dry smear of the cervical mucus and its accurate cyclical change is an interesting biological phenomenon and it has established a definite value in its clinical use as a diagnostic aid. Some other body fluids were also found to form similar crystallization.
Experiments by others showed that egg albumin and NaCl, when mixed and dried, also showed similar crystallization. This experiment was performed to investigate the sera, plasma and egg albumin, and salt solutions of various sorts to observe their effects in the formation of the crystallization.
Sera from men and women, and from. various animals, and plasma from men, and egg albumin were prepared. Salt solutions of various concentrations were made from NaCl, KCI, NaNO3, BaClh, KiSO4, KHSO4, NH4Br, K2Cr2O7, Na2SO4, K2CO3, FeCl2, Na3PO4,MgSO4 and SbzS5. The sera, plasma or egg albumin and salt solutions were mixed and dried on the slides at various temperatures and were observed under the microscope. The following results were obtained:
1) No crystal or crystallization was found on the dry smear of the sera, plasma or egg albumin alone.
2) Among the various kinds of salts, when their solutions were mixed with sera, plasma or egg albumin, NaNO3, BaClz, K2SO4, KHSO4, NH4Br, K2Cr2O7 showed crystallizations of their own characteristic patterns, but none was found with Na2SO4, K2C03, FeChz, Na3PO4, MgSo4, and Sb2S5.
3) The crystallization produced by NaCl and KCI solutions were most distinct and their patterns were nearly identical. They also closely resembled that of the arborization of the cervical mucus.
4) The pattern and size of the crystallization were different according to the concentration of the salt solutions. The maximum crystallization concentrations of the salt solutions were generally 10.0%. The oligo-crystallization concentrations were somewhat different according to the sera of various sorts, but in most cases no definite crystallization was recognized in concentrations below 3.0-1.0% salt solutions.
5) When the amount of salt solutions of NaC1 and KCI were increased for a constant amount of sera, the patterns of the crytallizations were changed and the size became, smaller and at last it showed a pattern of terrace formation.
6) Room temperature (20°C) was more adequate for the formation of crystallization comparing with that on a blood-typing viewer (50°C), or in the incubator (70°C) or on the direct flame.
7) No gross differences were found in the formation of crystallization and its pattern among sera, plasma and egg albumin.

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