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Estrogen의 化學的 側定法에 對한 檢討

A Study on the Chemical Determination of Urinary Estrogen

대한산부인과학회지 1965년 8권 3호 p.45 ~ 53
유용근,
소속 상세정보
유용근 (  ) - 한일병원 산부인과

Abstract


Determination of urinary estrogen is important to understand the physiology and diseases of the woman.
Estrgens are present in the urine in conjugated form, mainly as glucosiduronate and ester sulfates.
For the purpose of quantitative determination of urinary estrogen it is necessary to carry out an extensive concentration and purification.
This is usually effected by acid or enzymatic hydrolysis followed by extraction of the liberated estrogens with organic solvents.
But during the above procedures some loss of estnogen is inevitably followed.
It seems to be very difficult to introduce any specific method which could completely prevent loss of ´the estrogen.
Author deveolped a method which was relatively able to prevent loss of the estrogen and applied this to determination of estrogens of normal. or abnormal Women. Following results were obtained.
1) Acid hydrolysis with 15 vol. % HCI is more rapid and,simple than enzyme hydrolysis.
2). The ether is a most suitable solvent for extraction of liberated estrogen by hydrolysis and
carbonate buffer of pH 10.4 is used for purification of the other extracts.
3) The petroleum-ether is more adequate for the solvent of reextraction than ether and benzene.
4) Column chromatography is more simple than others.
5) For the colorimetry the hydroquinone-Kober method is more sensitive than Kober-Venning method.
6) Estrogen value for normally menstruating women is peak at secretory and ovulatory phases. Its mean value is 86.3 r/day.
7) Urinary estrogen value of amenorrheic women is relatively lower than that of normal women. Its mena value; 63.0x/24 hrs.
Climacteric women also shows lower level than that seen in normal women. It seems therefore that in both amenorrhea and climacterium more estradiol (than normal women) is converted to estrone and estriol.
8) In castrated women mean estrogen value is 46.2 r/24 hrs. and estradiol-estrone conversion rate is decreased.

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