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蛋白性호르몬의 免疫學的 硏究

Immunologic Studies of Proteohormones

대한산부인과학회지 1971년 14권 12호 p.23 ~ 28
소속 상세정보
申冕雨/Shin, Nyon Woo 柳長熙/姜詔子/Yu, Chang Hi/Kang, Jo Ja

Abstract


For the immunologic determination of H. C. G. in both urine and serum in the patients of hyperemesis gravidarum and the comparison of the concentration of H. C. G. in both serum and amniotic fluid of term pregnant women basic experiments and semi-quantitative assay has been performed by means of hemagglutination inhibition technique. The obtained results are as following.
1. H. C. G. of relatively high purity(5, 427 IU/mg.) was used as antigen and high titer of antiserum was obtained even with relatively small amount of antigen through booster injection of antigen and complete Freund´s adjuvant.
2. The specificity of the obtained antiserum has been confirmed by the neutralization test of biologic activity of H. C. G. on the weight of immature rat ovary. The analysis of the purity of H C. G. with Ouchterlony test revealed that the used H. C. G. - contained negligible amount of other proteins.
3. In the .patient of hyperemesis gravidarum, the titer of H C. G. in both serum and urine were significontly higher than that of normal pregnancy at the same period.
4. The severity of emetic symptoms seems relatively correlate with the titer of H. C. G. in both urine and serum. 5. The titer of H C. G. in both serum and amniotic fluid of 40 term pregnant women was 28.OIU/ml. 5.31U/ml. respeefively. The ratio of H C. G. concentration of amniotic fluid to serum was 17.3%.

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