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Toxoplasma gondii 의 조직배양에 관한 연구 Studies on the Toxoplama gondii in Buffy Coat Cell Culture

대한수의학회지 1973년 13권 1호 p.67 ~ 73
김종면,
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김종면 (  ) 
전북대학교 농과대학 수의학과

Abstract


Toxoplasma gondii (Tp), RH strain, was inoculated into cultured buffy coat cells obtained from the swine blood. The main reason for adopting swine lies in the animal’s unusual (susceptibility to Tp. As for the culture method used in the experiment, those well proved methods practised by Cho, Merchant, Moore and Tarnvik were mainly referred to as a starting point: hence, the author’s method has been turned out to be the modified or supplementary form of those methods. Observations were made on the phase of multiplication of Tp in the cytoplasm.
The results obtained were as follows :
1. Better growth and multiplication of Toxoplasma gondii were noticeably observed in the swine buffy coat cell, inoculated after three-to-five day cultivation of the cell.
2. In the lapse of the observation period, there appeared Toxoplasma gondii rarely available in the earlier stage, which had been inoculated into the cell after three-to-five day cultivation. In other words, Toxoplasma gondii started to show itself in seven or eight hours after inoculation, most outstandingly noticeable between twenty four hours and forty eight hours. Thereafter the disintegration stage of Toxoplasma gondii was observed.

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