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Comparative analysis of serological tests and fecal detection in the diagnosis of Mycobacterium avium subspecies paratuberculosis infection

대한수의학회지 2020년 60권 3호 p.117 ~ 122
박홍태, 하승민, 박현의, 심수진, 허태영, 유한상,
소속 상세정보
박홍태 ( Park Hong-Tae ) - Seoul National University College of Veterinary Medicine Department of Infectious Diseases
하승민 ( Ha Seung-Min ) - Rural Development Administration National Institute of Animal Science Department of Animal Resources Development
박현의 ( Park Hyun-Eui ) - Seoul National University College of Veterinary Medicine Department of Infectious Diseases
심수진 ( Shim Soo-Jin ) - Seoul National University College of Veterinary Medicine Department of Infectious Diseases
허태영 ( Hur Tai-Young ) - Rural Development Administration National Institute of Animal Science Department of Animal Resources Development
유한상 ( Yoo Han-Sang ) - Seoul National University College of Veterinary Medicine Department of Infectious Diseases

Abstract


Johne's disease (JD) caused by Mycobacterium avium subspecies paratuberculosis (MAP) is a chronic, wasting infectious disease in ruminants that causes enormous economic losses to the dairy and beef cattle industries. The most effective way to eradicate JD is to detect infected individuals as early as possible and remove them from the herd. However, it is difficult to detect infected individuals early with the currently using diagnostic methods. Two serological diagnostic kits commercially used worldwide and a fecal detection test were compared using 298 serum samples and feces of cattle in this study to present an efficient diagnostic method. Although there was a high correlation between the 2 serological diagnostic kits (R2 = 0.7473), kit A showed a higher serological positive rate. However, the correlation between fecal tests and serological diagnosis was very low. MAP was also detected in fecal tests in many serologically negative individuals. In the periodical diagnosis of JD, MAP was detected in the feces of only cows with the higher antibody titer to MAP. These results suggest that for effective eradication of JD, early detection of infected individuals by fecal tests together with the serological tests currently in use and by removal of infected individuals are needed.

키워드

paratuberculosis; diagnosis; enzyme-linked immunosorbent assay; polymerase chain reaction

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