잠시만 기다려 주세요. 로딩중입니다.

Effects of poly (ethylene glycol-propylene glycol) copolymer on hemostasis and osteogenesis in a rat calvarial defect model

대한수의학회지 2020년 60권 3호 p.145 ~ 153
김하은, 윤헌영, 김은진, 김선종,
소속 상세정보
김하은 ( Kim Ha-Eun ) - Konkuk University College of Veterinary Medicine Department of Veterinary Surgery
윤헌영 ( Yoon Hun-Young ) - Konkuk University College of Veterinary Medicine Department of Veterinary Surgery
김은진 ( Kim Eun-Jin ) - Theracion Biomedical
김선종 ( Kim Sun-Jong ) - Theracion Biomedical

Abstract


This study aimed to evaluate the effects of a bioabsorbable bone hemostatic agent comprising poly (ethylene glycolpropylene glycol) copolymers (PEG-PPG) on hemostasis and osteogenesis. Bilateral 3 mm diameter calvarial defects were created in 99 male Sprague-Dawley rats. The defects were filled with PEG-PPG or bone wax. The defects of control group were left unfilled. Virtual autopsy was performed to evaluate bioabsorption. The calvaria were subjected to x-ray microtomography (microCT) and histological examination. Bone volume fraction (BV/TV) and bone mineral density (BMD) were measured using microCT; furthermore, white blood cell count and histological examination were performed. After application of PEG-PPG and bone wax, immediate hemostasis was achieved. Autopsy revealed that PEG-PPG disappeared within 48 h at the application site; in contrast, bone wax remained until 12 weeks. The PEG-PPG and control groups showed significantly more osteogenesis than the bone wax group with respect to BV/TV and BMD at 3, 6, and 12 weeks (p < 0.05). Histology revealed that the bone wax group exhibited little bone formation with inflammation. In contrast, PEG-PPG and control groups showed significantly more qualitative osteogenesis than the bone wax group (p < 0.01). In conclusion, PEG-PPG showed immediate hemostasis and was absorbed to allow progressive osteogenesis.

키워드

bone; hemostasis; osteogenesis; rats; x-ray microtomography

원문 및 링크아웃 정보

등재저널 정보

KCI