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Alpha-Chymotrypsin ¹× TrypsinÀÇ Ê«÷ÍÊÇØ¯¿¡ ´ëÇÑ ß¿úª

The Effects of Alpha-Chymotrypsin and Trypsin on the Rabbit¢¥s Cornea

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Abstract


The posterior surface of the rabbit¢¥s cornea was scraped by a, spatula or incised by a cy totome, followed by injection of alpha-chymotrypsin, or trypsin into the anterior chamber. The concentration of enzymes used were 5mg/cc and 0 5mg/cc of alpha-chymotrypsin and 5.000 Anson unit/cc and 500unit/cc of trypsin. Three minutes after the introduction of enzymes into the anterior chamber, irrigation of the anterior chamber with normal saline was performed.
The cornea was examined macroscopically and biomicroscopically for about 3 weeks. Histologicai examination was done on the 3rd and the 11th post-operative days. Tile cornea was affected more intensively and extensively when the stroma was injured than when only the endothelium was inturned. Alpha-chymotrypsin was more toxic to the cornea than trypsin and led sometimes to a corneal perforation.

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